Yamashita Shin-ichi, Masuda Yoshiko, Matsumoto Katsutaka, Okumura Yasuhiro, Matsuzaki Hosei, Kurizaki Takashi, Haga Yoshio, Katafuchi Shigeru, Murayama Toshihiko, Ikei Satoshi, Kawahara Katsunobu
Department of Surgery, National Hospital Organization Kumamoto Medical Center, Kumamoto, Japan.
Ann Thorac Cardiovasc Surg. 2007 Aug;13(4):236-9.
Phosphatase of the regenerating liver (PRL)-3 protein tyrosine phosphatase gene is expressed in colon cancer metastasis. To investigate the role of this gene in metastatic lung cancer, we compared PRL-3 gene expression between primary cancers, metastatic lung cancer, and normal lung tissue.
Five metastatic tumor and normal samples from non-small cell lung cancer patients were obtained at the National Hospital Organization Kumamoto Medical Center (Kumamoto, Japan). For a quantitative evaluation of RNA expression by PCR, we used Taqman PCR methods.
Although PRL-3 gene expression levels in the primary lesions were slightly decreased compared with those in the normal tissues, those in the metastatic lesions were extremely down-regulated in the synchronous metastatic case. In 2 of these 3 cases, the metastatic tumors showed down-regulated PRL-3 gene expression at 10 times less than that of the normal tissue, and the other tumor showed a slightly weaker expression.
These data suggest that a down-regulation of the PRL-3 gene is important in lung cancer metastasis and provide a new hypothesis of lung cancer metastases.
再生肝磷酸酶（PRL）-3蛋白酪氨酸磷酸酶基因在结肠癌转移中表达。为了研究该基因在转移性肺癌中的作用，我们比较了原发性癌、转移性肺癌和正常肺组织中PRL-3基因的表达情况。
在日本熊本的国立医院组织熊本医疗中心获取了5例非小细胞肺癌患者的转移瘤和正常样本。为了通过PCR对RNA表达进行定量评估，我们使用了Taqman PCR方法。
虽然原发性病变中PRL-3基因的表达水平与正常组织相比略有下降，但在同步转移病例中，转移性病变中的表达极度下调。在这3例中的2例中，转移瘤中PRL-3基因的表达下调至正常组织的十分之一以下，另一例肿瘤的表达略弱。
这些数据表明PRL-3基因的下调在肺癌转移中很重要，并为肺癌转移提供了新的假说。
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