Glover K A, Grimholt U, Bakke H G, Nilsen F, Storset A, Skaala Ø
Institute of Marine Research, PO Box 1870, Nordnes, 5817 Bergen, Norway.
Dis Aquat Organ. 2007 Jun 7;76(1):57-65. doi: 10.3354/dao076057.
The relationship between genetic variation in major histocompatibility complex (MHC) Class I and II genes and susceptibility to sea lice Lepeophtheirus salmonis (Krøyer) in Atlantic salmon Salmo salar (L.) was studied in cage-reared post smolts. Polymorphic repeat markers located in the 3' untranslated regions (3UTR) of the genes Sasa-UBA (MHC Class I) and Sasa-DAA (MHC Class II) were screened in 1004 fish sampled from 11 full-sibling families. This gave rise to a total of 7 and 5 alleles, and 17 and 13 genotypes respectively. Significant relationships between both Sasa-UBA-3UTR and Sasa-DAA-3UTR genotypes and abundance of lice were observed within the pooled material, within individual families, and within the pooled material with both markers combined. However, most of these associations were either weak, linked with variation in fish size among genotypes, or influenced by family background genome. Nevertheless, within one family, the Sasa-DAA-3UTR 248/278 genotype displayed a significantly higher (33%) abundance of lice compared with the Sasa-DAA-3UTR 208/258 genotype, and this difference was not influenced by fish size. Consequently, the results of this study indicate a link between MHC Class II and susceptibility to lice.
在网箱养殖的大西洋鲑（Salmo salar, L.）降海幼鲑中，研究了主要组织相容性复合体（MHC）Ⅰ类和Ⅱ类基因的遗传变异与感染鲑海虱（Lepeophtheirus salmonis, Krøyer）易感性之间的关系。在从11个全同胞家系采集的1004尾鱼中，筛选了位于Sasa - UBA基因（MHCⅠ类）和Sasa - DAA基因（MHCⅡ类）3'非翻译区（3UTR）的多态性重复标记。分别产生了7个和5个等位基因，以及17种和13种基因型。在合并样本、各个家系以及同时使用两个标记的合并样本中，均观察到Sasa - UBA - 3UTR和Sasa - DAA - 3UTR基因型与海虱丰度之间存在显著关系。然而，这些关联大多较弱，与基因型间鱼体大小的差异有关，或受家系背景基因组的影响。尽管如此，在一个家系中，与Sasa - DAA - 3UTR 208/258基因型相比，Sasa - DAA - 3UTR 248/278基因型的海虱丰度显著更高（33%），且这种差异不受鱼体大小的影响。因此，本研究结果表明MHCⅡ类与海虱易感性之间存在联系。
