雌激素受体阳性和阴性乳腺癌中血管生成的定量分析。
Quantification of angiogenesis in estrogen receptor-positive and negative breast carcinoma.
作者信息
Parentes-Vieira Jb, Lopes-Costa Pv, Pires Cg, Dos Santos Ar, Pereira-Filho Jd, da Silva Bb
机构信息
Department of Gynecology, Federal University of Piauí, Teresina, Piauí, Brazil.
出版信息
Int Semin Surg Oncol. 2007 Aug 24;4:22. doi: 10.1186/1477-7800-4-22.
BACKGROUND
The objective of this study was to evaluate angiogenesis according to CD34 antigen expression in estrogen receptor (ER)-positive and negative breast carcinomas.
METHODS
This study comprised 64 cases of infiltrating ductal carcinoma in postmenopausal women divided into two groups: Group A: ER-positive, n = 35; and Group B: ER-negative, n = 29. The anti-CD34 monoclonal antibody was used as a marker for endothelial cells. Microvessel count was carried out in 10 fields per slide using a 40x objective lens (magnification 400x). Statistical analysis of the data was performed using Student's t-test (p < 0.05).
RESULTS
The mean number of vessels stained with the anti-CD34 antibody in the estrogen receptor-positive and negative tumors was 23.51 +/- 1.15 and 40.24 +/- 0.42, respectively. The number of microvessels was significantly greater in the estrogen receptor-negative tumors (p < 0.001).
CONCLUSION
ER-negative tumors have significantly greater CD34 antigen expression compared to ER-positive tumors.
背景
本研究的目的是根据雌激素受体（ER）阳性和阴性乳腺癌中CD34抗原的表达来评估血管生成情况。
方法
本研究纳入64例绝经后女性浸润性导管癌患者，分为两组：A组：ER阳性，n = 35；B组：ER阴性，n = 29。抗CD34单克隆抗体用作内皮细胞的标志物。使用40倍物镜（放大倍数400倍）在每张载玻片的10个视野中进行微血管计数。采用Student's t检验对数据进行统计学分析（p < 0.05）。
结果
雌激素受体阳性和阴性肿瘤中抗CD34抗体染色的血管平均数分别为23.51 +/- 1.15和40.24 +/- 0.42。雌激素受体阴性肿瘤中的微血管数量明显更多（p < 0.001）。
结论
与ER阳性肿瘤相比，ER阴性肿瘤的CD34抗原表达明显更高。
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