Erdem Ozlem, Dursun Aye, Coşkun Uğur, Günel Nazan
Department of Pathology, Faculty of Medicine, Gazi University, Ankara, Turkey.
Tumori. 2005 Jan-Feb;91(1):46-52. doi: 10.1177/030089160509100109.
p53, c-erbB-2 and Ki-67 protein expression and microvessel density (MVD) determined by CD34 antibody were evaluated by immunohistochemistry and their correlation with clinicopathological parameters including estrogen (ER) and progesterone (PR) receptor status and survival were investigated in patients with axillary lymph node-negative infiltrating ductal breast carcinoma.
The study population consisted of 47 patients with axillary lymph node-negative infiltrating ductal breast carcinoma.
p53 and c-erbB-2 expression was detected in 36.2% and 31.9% of patients, respectively. Median Ki-67 expression was 10%. There were no statistically significant differences in the distribution of p53, Ki-67 and c-erbB-2 protein expression in relation to the age of the patients or to the size, histological grade or ER and PR status of the tumors. p53 protein expression correlated positively with c-erbB-2 and Ki-67 protein expression (P < 0.05). The mean MVD was 63.65 +/- 29.1 and it correlated positively with histological grade and Ki-67 expression (P < 0.05). Survival analysis revealed that age, tumor size, p53 and c-erbB-2 expression and PR status had no significant prognostic impact, whereas histological grade, proliferative activity and angiogenic activity were significant prognostic factors. Although ER-positive patients had a statistically significant overall survival advantage, the difference in disease-free survival was not significant.
In axillary lymph node-negative breast carcinoma the histological grade and the proliferative and angiogenic activity of the tumor could be useful prognostic indicators.
采用免疫组织化学方法评估p53、c-erbB-2和Ki-67蛋白表达以及用CD34抗体测定的微血管密度（MVD），并研究其与腋窝淋巴结阴性浸润性导管癌患者临床病理参数（包括雌激素（ER）和孕激素（PR）受体状态）及生存率的相关性。
研究人群包括47例腋窝淋巴结阴性浸润性导管癌患者。
分别在36.2%和31.9%的患者中检测到p53和c-erbB-2表达。Ki-67表达中位数为10%。p53、Ki-67和c-erbB-2蛋白表达的分布在患者年龄、肿瘤大小、组织学分级或ER和PR状态方面无统计学显著差异。p53蛋白表达与c-erbB-2和Ki-67蛋白表达呈正相关（P<0.05）。平均MVD为63.65±29.1，且与组织学分级和Ki-67表达呈正相关（P<0.05）。生存分析显示，年龄、肿瘤大小、p53和c-erbB-2表达及PR状态无显著预后影响，而组织学分级、增殖活性和血管生成活性是显著的预后因素。尽管ER阳性患者总体生存有统计学显著优势，但无病生存差异不显著。
在腋窝淋巴结阴性乳腺癌中，肿瘤的组织学分级以及增殖和血管生成活性可能是有用的预后指标。
