Förster C, Brauer A B E, Fürste J P, Betzel Ch, Weber M, Cordes F, Erdmann V A
Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany.
Biochem Biophys Res Commun. 2007 Oct 19;362(2):415-8. doi: 10.1016/j.bbrc.2007.07.178. Epub 2007 Aug 10.
Aminoacyl-tRNA synthetases catalyze the formation of aminoacyl-tRNAs. Seryl-tRNA synthetase is a class II synthetase, which depends on rather few and simple identity elements in tRNA(Ser) to determine the amino acid specificity. tRNA(Ser) acceptor stem microhelices can be aminoacylated with serine, which makes this part of the tRNA a valuable tool for investigating the structural motifs in a tRNA(Ser)-seryl-tRNA synthetase complex. A 1.8A-resolution tRNA(Ser) acceptor stem crystal structure was superimposed to a 2.9A-resolution crystal structure of a tRNA(Ser)-seryl-tRNA synthetase complex for a visualization of the binding environment of the tRNA(Ser) microhelix.
氨酰-tRNA合成酶催化氨酰-tRNA的形成。丝氨酰-tRNA合成酶是一种II类合成酶,它依赖于tRNA(Ser)中相当少且简单的识别元件来确定氨基酸特异性。tRNA(Ser)受体茎微螺旋可以被丝氨酸氨酰化,这使得tRNA的这一部分成为研究tRNA(Ser)-丝氨酰-tRNA合成酶复合物中结构基序的有价值工具。将分辨率为1.8埃的tRNA(Ser)受体茎晶体结构与分辨率为2.9埃的tRNA(Ser)-丝氨酰-tRNA合成酶复合物晶体结构进行叠加,以可视化tRNA(Ser)微螺旋的结合环境。