人丝氨酰 - tRNA合成酶的最小tRNA(Ser)和tRNA(Sec)底物：tRNA结构域对丝氨酰化和三级结构的贡献

Minimal tRNA(Ser) and tRNA(Sec) substrates for human seryl-tRNA synthetase: contribution of tRNA domains to serylation and tertiary structure.

作者信息

Heckl M, Busch K, Gross H J

机构信息

Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Biozentrum, Würzburg, Germany.

出版信息

FEBS Lett. 1998 May 15;427(3):315-9. doi: 10.1016/s0014-5793(98)00435-9.

DOI:10.1016/s0014-5793(98)00435-9

PMID:9637248

Abstract

The recognition process of tRNA(Ser) and tRNA(Sec) by human seryl-tRNA synthetase (SerRS) was studied using T7 transcripts representing defined regions of human tRNA(Ser) or tRNA(Sec) and the influence of the tRNA elements on serylation and tertiary structure was elucidated. The anticodon arms of both tRNAs showed no contribution to serylation in contrast to the acceptor stems and the long extra arms. D and T arms were only involved in formation of the L-shaped tRNA structure, not in the recognition process between tRNAs and SerRS. This is the first report of microhelices adapted from human tRNAs being aminoacylated by their homologous synthetase.

摘要

利用代表人类tRNA(Ser)或tRNA(Sec)特定区域的T7转录本，研究了人丝氨酰-tRNA合成酶（SerRS）对tRNA(Ser)和tRNA(Sec)的识别过程，并阐明了tRNA元件对丝氨酰化和三级结构的影响。与受体茎和长的额外臂相比，两种tRNA的反密码子臂对丝氨酰化均无贡献。D臂和T臂仅参与L形tRNA结构的形成，而不参与tRNA与SerRS之间的识别过程。这是关于源自人类tRNA的微螺旋被其同源合成酶氨酰化的首次报道。