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用于分离人类精子的上游法和玻璃珠柱法之间的比较。

Comparison between swim-up and glass bead column techniques for the separation of human spermatozoa.

作者信息

Calamera J C, Quiros M C, Brugo S, Nicholson R F

机构信息

L.E.R., Laboratorio de Estudios en Reproduccion, Centro de Estudios en Ginecologia y Reproduction, Buenos Aires, Argentina.

出版信息

Andrologia. 1991 Jul-Aug;23(4):259-61. doi: 10.1111/j.1439-0272.1991.tb02554.x.

DOI:10.1111/j.1439-0272.1991.tb02554.x
PMID:1772137
Abstract

The glass bead column filtration method was compared with the swim-up technique regarding spermatozoa recovery in normal and pathological human semen. The general characteristics of the semen and the spermatic ATP concentration were measured in 67 patients. Samples processed with glass bead columns provided a good amount of motile forms which however, showed less velocity and linearity than in the case of using the swim-up technique. Additionally, the ATP determination in spermatozoa was included.

摘要

在正常和病理性人类精液中,就精子回收率而言,对玻璃珠柱过滤法与上游技术进行了比较。对67例患者的精液一般特征和精子ATP浓度进行了测量。用玻璃珠柱处理的样本提供了大量活动形态的精子,然而,与使用上游技术的情况相比,其速度和直线性较低。此外,还进行了精子中的ATP测定。

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