Gal A E, Fash F J
Chem Phys Lipids. 1976 Feb;16(1):71-9. doi: 10.1016/0009-3084(76)90015-3.
2-N-(Hexadecanoyl)-amino-4-nitrophenyl phosphorylcholine-hydroxide a compound resembling sphingomyelin is synthesized. It is cleaved by sphingomyelinase to the chromogenic N-acylaminonitrophenyl moiety. Phospholipase C preparations do not hydrolyze this compound. The starting material is 2-amino-4-nitrophenol which when acylated with palmitoyl chloride yields the hexadecananilide. Reaction with beta-bromoethylphosphoryldichloride gives the phosphate which is quaternized with trimethylamine to give the title compound.
合成了一种类似于鞘磷脂的化合物2-N-（十六烷酰基）-氨基-4-硝基苯基氢氧化磷酰胆碱。它被鞘磷脂酶裂解为发色性N-酰基氨基硝基苯基部分。磷脂酶C制剂不会水解该化合物。起始原料是2-氨基-4-硝基苯酚，其与棕榈酰氯酰化后生成十六烷酰苯胺。与β-溴乙基磷酰二氯反应得到磷酸盐，该磷酸盐用三甲胺季铵化得到标题化合物。
