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肝星状细胞中电压依赖性钾离子电流的异质性组成

Heterogeneous composition of voltage-dependent K(+) currents in hepatic stellate cells.

作者信息

Lee Dong Hyeon, Kimm Kuchan, Kim Hyung-Lae, Han Bok Ghee

机构信息

Division of Biobank for Health Sciences, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, 194 Tongil-ro, Eunpyung-gu, Seoul 122-701, Korea.

出版信息

Yonsei Med J. 2007 Aug 31;48(4):684-93. doi: 10.3349/ymj.2007.48.4.684.

DOI:10.3349/ymj.2007.48.4.684
PMID:17722243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2628052/
Abstract

PURPOSE

Hepatic stellate cells (HSC) are a type of pericyte with varying characteristics according to their location. However, the electrophysiological properties of HSC are not completely understood. Therefore, this study investigated the difference in the voltage-dependent K(+) currents in HSC.

MATERIALS AND METHODS

The voltage-dependent K(+) currents in rat HSC were evaluated using the whole cell configuration of the patch-clamp technique.

RESULTS

Four different types of voltage-dependent K(+) currents in HSC were identified based on the outward and inward K(+) currents. Type D had the dominant delayed rectifier K(+) current, and type A had the dominant transient outward K(+) current. Type I had an inwardly rectifying K(+) current, whereas the non-type I did not. TEA (5 mM) and 4-AP (2 mM) suppressed the outward K(+) currents differentially in type D and A. Changing the holding potential from -80 to -40 mV reduced the amplitude of the transient outward K(+) currents in type A. The inwardly rectifying K(+) currents either declined markedly or were sustained in type I during the hyperpolarizing step pulses from -120 to -150 mV.

CONCLUSION

There are four different configurations of voltage-dependent K(+) currents expressed in cultured HSC. These results are expected to provide information that will help determine the properties of the K(+) currents in HSC as well as the different type HSC populations.

摘要

目的

肝星状细胞（HSC）是一种周细胞，根据其位置具有不同特征。然而，HSC的电生理特性尚未完全了解。因此，本研究调查了HSC中电压依赖性钾离子电流的差异。

材料与方法

采用膜片钳技术的全细胞模式评估大鼠HSC中的电压依赖性钾离子电流。

结果

根据外向和内向钾离子电流，在HSC中鉴定出四种不同类型的电压依赖性钾离子电流。D型具有占主导地位的延迟整流钾离子电流，A 型具有占主导地位的瞬时外向钾离子电流。I型具有内向整流钾离子电流，而非I型则没有。TEA（5 mM）和4-AP（2 mM）对D型和A型的外向钾离子电流有不同程度的抑制作用。将钳制电位从-80 mV 改变为-40 mV 可降低 A 型瞬时外向钾离子电流的幅度。在从-120 mV 到-150 mV 的超极化阶跃脉冲期间，I型的内向整流钾离子电流要么显著下降，要么持续存在。

结论

在培养的HSC中表达有四种不同构型的电压依赖性钾离子电流。这些结果有望提供有助于确定HSC中钾离子电流特性以及不同类型HSC群体的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/8b7f3dccf1de/ymj-48-684-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/199ede2d05f7/ymj-48-684-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/8aa2677c10d1/ymj-48-684-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/03119e8c208c/ymj-48-684-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/1de5f41853c5/ymj-48-684-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/97c2b67c06d6/ymj-48-684-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/8b7f3dccf1de/ymj-48-684-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/199ede2d05f7/ymj-48-684-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/8aa2677c10d1/ymj-48-684-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/03119e8c208c/ymj-48-684-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/1de5f41853c5/ymj-48-684-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/97c2b67c06d6/ymj-48-684-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2348/2628052/8b7f3dccf1de/ymj-48-684-g006.jpg

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Human myofibroblastic hepatic stellate cells express Ca(2+)-activated K(+) channels that modulate the effects of endothelin-1 and nitric oxide.
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