Khang Dongwoo, Sato Michiko, Price Rachel L, Ribbe Alexander E, Webster Thomas J
Department of Physics, Purdue University, West Lafayette, IN, USA.
Int J Nanomedicine. 2006;1(1):65-72. doi: 10.2147/nano.2006.1.1.65.
In an effort to develop better orthopedic implants, osteoblast (bone-forming cells) adhesion was determined on microscale patterns (30 microm lines) of carbon nanofibers placed on polymer substrates. Patterns of carbon nanofibers (CNFs) on a model polymer (polycarbonate urethane [PCU]) were developed using an imprinting method that placed CNFs in selected regions. Results showed the selective adhesion and alignment of osteoblasts on CNF patterns placed on PCU. Results also showed greater attraction forces between fibronectin and CNF (compared with PCU) patterns using atomic force microscope force-displacement curves. Because fibronectin is a protein that mediates osteoblast adhesion, these results provide a mechanism of why osteoblast adhesion was directed towards CNF patterns. Lastly, this study showed that the directed osteoblast adhesion on CNF patterns translated to enhanced calcium phosphate mineral deposition along linear patterns of CNFs on PCU. Since CNFs are conductive materials, this study formulated substrates that through electrical stimulation could be used in future investigations to further promote osteoblasts to deposit anisotropic patterns of calcium-containing mineral similar to that observed in long bones.
为了开发更好的骨科植入物，研究人员测定了成骨细胞（骨形成细胞）在置于聚合物基质上的碳纳米纤维微观图案（30微米线条）上的粘附情况。使用一种将碳纳米纤维放置在选定区域的压印方法，在模型聚合物（聚碳酸酯聚氨酯[PCU]）上制作了碳纳米纤维图案。结果显示，成骨细胞在置于PCU上的碳纳米纤维图案上有选择性的粘附和排列。研究结果还显示，使用原子力显微镜力-位移曲线，与PCU相比，纤连蛋白和碳纳米纤维图案之间的吸引力更大。由于纤连蛋白是一种介导成骨细胞粘附的蛋白质，这些结果提供了成骨细胞粘附指向碳纳米纤维图案的机制。最后，这项研究表明，成骨细胞在碳纳米纤维图案上的定向粘附转化为沿PCU上碳纳米纤维线性图案增强的磷酸钙矿物质沉积。由于碳纳米纤维是导电材料，这项研究设计了一些基质，通过电刺激，这些基质可用于未来的研究，以进一步促进成骨细胞沉积与长骨中观察到的类似的含钙质矿物质的各向异性图案。
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