感染路氏锥虫并用环磷酰胺治疗的大鼠体内α2巨球蛋白活性及其对疾病恶性程度的影响。

Alpha 2 macroglobulin activity in rats infected with Typanosoma lewisi and treated with cyclophosphamide and its effect on the malignancy of the disease.

作者信息

Budovsky A, Sneir R, Bazarsky E, El-On J

机构信息

Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

出版信息

J Vector Borne Dis. 2007 Jun;44(2):128-36.

PMID:17722867

Abstract

BACKGROUND & OBJECTIVES: Trypanosoma lewisi is a common, flagellated parasite of the rat. Our previous study showed that rabbits injected with serum collected from rats infected with Trypanosoma lewisi and treated with cyclophosphamide (CyI) produced high levels of antibodies against a new protein in the CyI rat serum.

RESULTS

In the present study, this protein was characterised as alpha2 macroglobulin (alpha2M) and the kinetics of its production and its influence on the malignancy of the disease were determined. In rats infected with T. lewisi, alpha2M was first demonstrated and peaked on the second day post-infection (972 microg/ml) and then reduced gradually, reaching a level of 32 microg/ml on the eighth day post-infection. However, in the CyI rats the level of alpha2M was gradually increased as the disease progressed, reaching a level of 890 microg/ml on the eighth day post-infection. Injection of both crude and purified alpha2M into rats infected with T. lewisi led to increased parasitaemia.

INTERPRETATION & CONCLUSION: The present study suggests that increased levels of alpha2M in the CyI rats contribute to the malignancy of the disease.

摘要

背景与目的

路氏锥虫是大鼠常见的一种有鞭毛的寄生虫。我们之前的研究表明，给兔子注射从感染路氏锥虫并经环磷酰胺（CyI）处理的大鼠采集的血清后，兔子产生了针对CyI大鼠血清中一种新蛋白质的高水平抗体。

结果

在本研究中，这种蛋白质被鉴定为α2巨球蛋白（α2M），并确定了其产生的动力学及其对疾病恶性程度的影响。在感染路氏锥虫的大鼠中，首次检测到α2M，在感染后第二天达到峰值（972微克/毫升），然后逐渐降低，在感染后第八天降至32微克/毫升。然而，在CyI大鼠中，随着疾病进展，α2M水平逐渐升高，在感染后第八天达到890微克/毫升。将粗制和纯化的α2M注射到感染路氏锥虫的大鼠中会导致寄生虫血症增加。