Carrera Nivia J Ríos, Carmona Misael Chinchilla, Guerrero Olga M, Castillo Alfredo Castro
Departamento de Parasitología, Facultad de Microbiología, Universidad de Costa Rica, Costa Rica.
Rev Biol Trop. 2009 Mar-Jun;57(1-2):13-22.
The immunosuppressant effect of T. lewisi infection on the multiplication of T. gondii was compared in peritoneal (MP) and alveolar macrophages (MA) of white rat. Two animal groups were infected with T. lewisi and sacrificed after four days and seven days post infection. A group without infection was maintained as a control. The number of intracellular parasites (tachyzoites) (IT) was counted by light microscopy, calculating the rate infection rate per 100 total cells (TC) and per infected cells (IC) for each group of phagocyte cells. The relation quotient IT, TC or IC multiplied percent, provided a statistical ratio (RE) of the relative number of parasites in both cellular types for each time interval. MA as well as MP obtained after 4 days showed a significant increase in the multiplication of T. gondii with respect to the control. Unlike the MP (which had an increase in the multiplication of T. gondii the fourth day of infection with T. lewisi diminishing towards the seventh day), the MA had an increase in the multiplication of the parasite from the fourth to the seventh day. This difference can be related to the route of infection used for the experiments, that affect the MP directly with a greater effect in comparison with the MA of the lungs. Lung compartment will be affected later, when the infection becomes systemic between the fourth and sixth day of infection. The immunity against T. gondii is similar between both phagocytes, but the time of infection and the compartment where the cells are located, makes the difference in the response time against T. gondii. Supernatants from macrophage cultures or T. lewisi by rat did not induced any immunosuppression.
在白鼠的腹腔巨噬细胞（MP）和肺泡巨噬细胞（MA）中比较了路易氏锥虫感染对刚地弓形虫增殖的免疫抑制作用。将两组动物感染路易氏锥虫，并在感染后四天和七天处死。将一组未感染的动物作为对照。通过光学显微镜计数细胞内寄生虫（速殖子）（IT）的数量，计算每组吞噬细胞每100个总细胞（TC）和每感染细胞（IC）的感染率。IT、TC或IC乘以百分比的关系商提供了每个时间间隔两种细胞类型中寄生虫相对数量的统计比率（RE）。与对照相比，感染四天后获得的MA以及MP中刚地弓形虫的增殖均显著增加。与MP不同（MP在感染路易氏锥虫后的第四天刚地弓形虫增殖增加，到第七天则减少），MA中寄生虫的增殖从第四天到第七天持续增加。这种差异可能与实验中使用的感染途径有关，与肺部的MA相比，感染途径对MP的直接影响更大。当感染在感染后的第四至第六天变为全身性时，肺部隔室随后会受到影响。两种吞噬细胞对刚地弓形虫的免疫力相似，但感染时间和细胞所在的隔室使得对刚地弓形虫的反应时间有所不同。大鼠巨噬细胞培养物或路易氏锥虫的上清液未诱导任何免疫抑制。
