Foresta C, Caretto A, Varotto A, Rossato M, Scandellari C
III Cattedra of Medical Pathology, University of Padua, Italy.
Arch Androl. 1991 Jul-Aug;27(1):17-24. doi: 10.3109/01485019108987647.
An indirect immunofluorescence technique was used to detect and localize epidermal growth factor receptors (EGFR) in human testicular biopsies in different testicular diseases. Monolateral biopsies from twelve infertile subjects were studied from qualitative/quantitative points of view and were examined by immunofluorescence study with anti-EGFR monoclonal antibody. Two different patterns of EGFR expression were observed: a very weak presence of EGFR was detectable on Sertoli cells, peritubular basal structures, and interstitial compartment in testicular biopsies showing a normal spermatogenic status; and an important increase in EGFR expression was observed on the cytoplasm of Sertoli cells and on peritubular basal structures in biopsies exhibiting various degrees of hypospermatogenesis and in a case of Sertoli cells only syndrome. Germ cells did not show EGFR immunolocalization. EGFR seems to be present on different testicular target cells. EGFR expression increases on peritubular and Sertoli cells in the presence of significant tubular damage.
采用间接免疫荧光技术检测并定位不同睾丸疾病患者睾丸活检组织中的表皮生长因子受体（EGFR）。从定性/定量角度研究了12例不育患者的单侧活检组织，并用抗EGFR单克隆抗体进行免疫荧光研究。观察到两种不同的EGFR表达模式：在生精状态正常的睾丸活检组织中，支持细胞、睾丸基底膜结构和间质区可检测到非常微弱的EGFR；在表现出不同程度精子发生减少的活检组织以及仅支持细胞综合征的病例中，支持细胞胞质和睾丸基底膜结构上的EGFR表达显著增加。生殖细胞未显示EGFR免疫定位。EGFR似乎存在于不同的睾丸靶细胞上。在存在明显的生精小管损伤时，睾丸基底膜和支持细胞上的EGFR表达增加。
