基于砷（III）-水杨酸体系荧光增强对水杨酸的分析。
Analysis of salicylic acid based on the fluorescence enhancement of the As(III)-salicylic acid system.
作者信息
Karim Mohammad Mainul, Lee Hwa Suk, Kim Young Sun, Bae Hyun Sook, Lee Sang Hak
机构信息
Department of Chemistry, Kyungpook National University, Taegu 702-701, Republic of Korea.
出版信息
Anal Chim Acta. 2006 Aug 18;576(1):136-9. doi: 10.1016/j.aca.2006.02.004. Epub 2006 Mar 7.
A new, simple and sensitive spectrofluorimetric method for the determination of salicylic acid (lambda(ex)=315 nm, lambda(em) = 408 nm) using As(III) as a sensitizing reagent has been investigated by measuring the increase of fluorescence intensity of salicylic acid due to the complexation of As(III)-salicylic acid in presence of sodium dodecyl sulfate (SDS) 10(-3) M. Under optimum conditions, a significant relationship was obtained between the fluorescence intensity and salicylic acid concentration. A linear calibration curve was obtained in the range 13.8-13812 microg l(-1) with product-moment correlation coefficient (R) 0.99985 and detection limit 4.2 microg l(-1). The R.S.D. is 2.35% (n=5). The method was applied successfully to the determination of salicylic acid in human serum.
研究了一种新的、简单且灵敏的荧光分光光度法，该方法以As(III)作为增敏试剂来测定水杨酸（激发波长λ(ex)=315 nm，发射波长λ(em)=408 nm）。通过测量在10(-3) M十二烷基硫酸钠（SDS）存在下，由于As(III)与水杨酸络合导致水杨酸荧光强度增加来进行测定。在最佳条件下，荧光强度与水杨酸浓度之间存在显著关系。在13.8 - 13812 μg l(-1)范围内获得线性校准曲线，乘积矩相关系数（R）为0.99985，检测限为4.2 μg l(-1)。相对标准偏差（R.S.D.）为2.35%（n = 5）。该方法成功应用于人血清中水杨酸的测定。
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