Han Yunxiao, Wu Xia, Yang Jinghe, Sun Shuna
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
J Pharm Biomed Anal. 2005 Jul 1;38(3):528-31. doi: 10.1016/j.jpba.2005.01.033.
In this paper, a simple, rapid and sensitive spectrofluorimetric method based on the formation of yttrium complexes has been developed for the determination of norfloxacin (NFLX). The affecting factors of the enhancement were studied in detail. Under optimum conditions, a linear relationship was obtained between the enhanced fluorescence intensity and the NFLX concentration in the range 1.0 x 10(-9) to 1.0 x 10(-5)mol/l and the detection limit of NFLX was 3.8 x 10(-11)mol/l (S/N=3). The method is applied for the determination of NFLX in actual sample (norfloxcain eye drops), the average recovery is 102.4% (n=5), and the result obtained is satisfactory.
本文建立了一种基于钇配合物形成的简单、快速、灵敏的荧光光谱法测定诺氟沙星（NFLX）。详细研究了增强作用的影响因素。在最佳条件下，增强荧光强度与NFLX浓度在1.0×10⁻⁹至1.0×10⁻⁵mol/L范围内呈线性关系，NFLX的检测限为3.8×10⁻¹¹mol/L（S/N = 3）。该方法用于实际样品（诺氟沙星滴眼液）中NFLX的测定，平均回收率为102.4%（n = 5），结果令人满意。
