短期油酰雌酮治疗会影响大鼠脂肪组织中脂质的管理能力。
Short-term oleoyl-estrone treatment affects capacity to manage lipids in rat adipose tissue.
作者信息
Salas Anna, Noé Véronique, Ciudad Carlos J, Romero M Mar, Remesar Xavier, Esteve Montserrat
机构信息
Departament de Nutrició i Bromatologia, Facultat de Biologia, Universitat de Barcelona, Av, Diagonal 645, 08028 Barcelona, Spain.
出版信息
BMC Genomics. 2007 Aug 28;8:292. doi: 10.1186/1471-2164-8-292.
BACKGROUND
Short-term OE (oleoyl-estrone) treatment causes significant decreases in rat weight mainly due to adipose tissue loss. The aim of this work was to determine if OE treatment affects the expression of genes that regulate lipid metabolism in white adipose tissue.
RESULTS
Gene expression in adipose tissue from female treated rats (48 hours) was analysed by hybridization to cDNA arrays and levels of specific mRNAs were determined by real-time PCR. Treatment with OE decreased the expression of 232 genes and up-regulated 75 other genes in mesenteric white adipose tissue. The use of real-time PCR validate that, in mesenteric white adipose tissue, mRNA levels for Lipoprotein Lipase (LPL) were decreased by 52%, those of Fatty Acid Synthase (FAS) by 95%, those of Hormone Sensible Lipase (HSL) by 32%, those of Acetyl CoA Carboxylase (ACC) by 92%, those of Carnitine Palmitoyltransferase 1b (CPT1b) by 45%, and those of Fatty Acid Transport Protein 1 (FATP1) and Adipocyte Fatty Acid Binding Protein (FABP4) by 52% and 49%, respectively. Conversely, Tumour Necrosis Factor (TNFalpha) values showed overexpression (198%).
CONCLUSION
Short-term treatment with OE affects adipose tissue capacity to extract fatty acids from lipoproteins and to deal with fatty acid transport and metabolism.
背景
短期油酰雌酮（oleoyl-estrone，OE）治疗会导致大鼠体重显著下降，主要原因是脂肪组织减少。本研究的目的是确定OE治疗是否会影响白色脂肪组织中调节脂质代谢的基因表达。
结果
通过与cDNA阵列杂交分析经OE处理的雌性大鼠（48小时）脂肪组织中的基因表达，并通过实时PCR测定特定mRNA的水平。OE处理可使肠系膜白色脂肪组织中232个基因的表达降低，另外75个基因上调。实时PCR验证，在肠系膜白色脂肪组织中，脂蛋白脂肪酶（Lipoprotein Lipase，LPL）的mRNA水平降低了52%，脂肪酸合酶（Fatty Acid Synthase，FAS）降低了95%，激素敏感性脂肪酶（Hormone Sensible Lipase，HSL）降低了32%，乙酰辅酶A羧化酶（Acetyl CoA Carboxylase，ACC）降低了92%，肉碱棕榈酰转移酶1b（Carnitine Palmitoyltransferase 1b，CPT1b）降低了45%，脂肪酸转运蛋白1（Fatty Acid Transport Protein 1，FATP1）和脂肪细胞脂肪酸结合蛋白（Adipocyte Fatty Acid Binding Protein，FABP4）分别降低了52%和49%。相反地，肿瘤坏死因子（Tumour Necrosis Factor，TNFalpha）的值显示过表达（198%）。
结论
短期OE治疗会影响脂肪组织从脂蛋白中提取脂肪酸以及处理脂肪酸转运和代谢的能力。
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