Nwodo N J, Brun R, Osadebe P O
Department of Pharmaceutical Chemistry, University of Nigeria, Nsukka, Nigeria.
J Ethnopharmacol. 2007 Sep 25;113(3):556-9. doi: 10.1016/j.jep.2007.07.018. Epub 2007 Jul 22.
The aqueous extract of young leaves of Holarrhena africana, a plant used in the Nigerian traditional medicine, exhibited good activity against Trypanosoma brucei spp. The extract was fractionated and eight fractions were obtained. One fraction designated as HaF(5) showed in vitro activity against Trypanosoma brucei rhodesiense with an IC(50) value of 0.785 microg/mg and no overt cytotoxicity against L-6 cells. Fraction HaF(5) was tested in vivo at two doses and found to exhibit in vivo efficacy in Trypanosoma brucei brucei infected mice leading to a complete disappearance of parasitaemia followed by a relapse.
非洲止泻木是一种用于尼日利亚传统医学的植物，其幼叶的水提取物对布氏锥虫表现出良好的活性。对该提取物进行了分级分离，得到了八个级分。其中一个名为HaF(5)的级分对罗德西亚布氏锥虫具有体外活性，IC(50)值为0.785微克/毫克，并且对L-6细胞没有明显的细胞毒性。对HaF(5)级分进行了两种剂量的体内试验，发现在感染布氏布氏锥虫的小鼠中具有体内疗效，导致寄生虫血症完全消失，随后复发。
