Bizimana Nsekuye, Tietjen Uwe, Zessin Karl-Hans, Diallo Drissa, Djibril Coulibaly, Melzig Matthias F, Clausen Peter-Henning
Institute for Parasitology und International Animal Health, Freie Universität Berlin, Königsweg 67, D-l4163 Berlin, Germany.
J Ethnopharmacol. 2006 Feb 20;103(3):350-6. doi: 10.1016/j.jep.2005.08.023. Epub 2005 Sep 26.
Water, methanol and dichloromethane extracts prepared from various parts of 40 medicinal plant species from Mali were investigated for their trypanocidal activity against Trypanosoma brucei brucei. Of a total of 165 extracts tested in vitro in the Low Inoculation Long Incubation Test (LILIT), 24 extracts showed a high trypanocidal activity. Using the Long-Term Viability Assay (LtVA) for corroboration of the results of the 24 extracts, it was found that 15 samples had minimum inhibitory concentration (MIC) values > 10 microg/ml, eight MIC values of 100 microg/ml and one MIC values of 50-100 microg/ml. So far, four extracts with MIC values < or = 100 microg/ml were tested for antitrypanosomal activity in mice, experimentally infected with Trypanosoma brucei brucei. Only, the aqueous extracts of the leaves of Terminalia avicennioides Guill. and Perr. (Combretaceae) and the stem bark of Ceiba pentandra (L.) Gaertn. (Bombacaceae) were able to reduce the parasitaemia in animals treated at the dose of 100 mg/kg b.w. (intraperitoneally, two times daily for 3 days) and of 150 mg/kg b.w. (per os, two times daily for 3 days), respectively. The reduction of parasitaemia was, however, statistically significant (p=0.002) only in case of treatment with Terminalia avicennioides.
对来自马里的40种药用植物不同部位制备的水提取物、甲醇提取物和二氯甲烷提取物进行了抗布氏锥虫锥虫杀灭活性研究。在低接种量长期培养试验（LILIT）中对总共165种提取物进行体外测试，其中24种提取物显示出高锥虫杀灭活性。使用长期活力测定法（LtVA）对这24种提取物的结果进行确证，发现15个样品的最低抑菌浓度（MIC）值>10微克/毫升，8个MIC值为100微克/毫升，1个MIC值为50 - 100微克/毫升。到目前为止，对4种MIC值≤100微克/毫升的提取物在实验感染布氏锥虫的小鼠中进行了抗锥虫活性测试。只有诃子Terminalia avicennioides Guill. and Perr.（使君子科）叶片的水提取物以及吉贝Ceiba pentandra (L.) Gaertn.（木棉科）茎皮的水提取物，分别在以100毫克/千克体重（腹腔注射，每日两次，共3天）和150毫克/千克体重（口服，每日两次，共3天）的剂量处理动物时能够降低寄生虫血症。然而，只有在用诃子处理的情况下，寄生虫血症的降低才具有统计学意义（p = 0.002）。
