Wojtczak Mariola, Dietrich Grzegorz J, Irnazarow Ilgiz, Jurecka Patrycja, Słowińska Mariola, Ciereszko Andrzej
Semen Biology Group, Institute of Animal Reproduction and Food Research, 10-747 Olsztyn, ul. Tuwima 10, Poland.
Comp Biochem Physiol B Biochem Mol Biol. 2007 Dec;148(4):426-31. doi: 10.1016/j.cbpb.2007.07.011. Epub 2007 Aug 1.
Transferrin (Tf) is a major protein of carp (Cyprinus carpio) seminal plasma. Its relationship with milt quality is unknown. In this study, we sought to determine if Tf is polymorphic in carp seminal plasma and if this polymorphism is related to sperm motility characteristics. We screened males of purebred common carp line (Polish line R6) for Tf polymorphism in blood plasma. The majority of Tf genotypes represented only DD and DG variants. We then collected milt from preselected DD and DG genotypes and tested their sperm motility characteristics using computer-aided sperm analysis (CASA). Tf polymorphism in seminal plasma was found to be identical with that of blood. However, the relationships between Tf polymorphism and iron metabolic parameters were different for blood and semen. These data suggest different regulation of Tf in liver and testis. We found substantial differences in sperm motility characteristics between both genotypes. Spermatozoa of DG males were characterized by lower curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), higher linearity (LIN) and straightness (STR) of movement as compared to DD males. No differences were found in other sperm characteristics such as sperm concentration and percentage of sperm motility. Our results suggest that sperm motility parameters are related to Tf polymorphism and therefore this polymorphism may be related to sperm competitive ability.
转铁蛋白（Tf）是鲤鱼（Cyprinus carpio）精浆中的一种主要蛋白质。其与精液质量的关系尚不清楚。在本研究中，我们试图确定Tf在鲤鱼精浆中是否具有多态性，以及这种多态性是否与精子活力特征相关。我们筛选了纯种鲤鱼品系（波兰品系R6）雄性个体血浆中的Tf多态性。大多数Tf基因型仅表现为DD和DG变体。然后，我们从预先选择的DD和DG基因型个体中采集精液，并使用计算机辅助精子分析（CASA）检测其精子活力特征。发现精浆中的Tf多态性与血液中的相同。然而，血液和精液中Tf多态性与铁代谢参数之间的关系有所不同。这些数据表明肝脏和睾丸中Tf的调控不同。我们发现两种基因型之间精子活力特征存在显著差异。与DD雄性个体相比，DG雄性个体的精子具有较低的曲线速度（VCL）、头部侧向位移幅度（ALH），较高的运动线性度（LIN）和直线度（STR）。在其他精子特征如精子浓度和精子活力百分比方面未发现差异。我们的结果表明精子活力参数与Tf多态性相关，因此这种多态性可能与精子竞争能力有关。
