Shi Yi-Chao, Shang Xue-Jun, Wang Xiu-Lai, Huang Yu-Feng
Department of Andrology, School of Clinical Medicine, Nanjing University Medical College/Nanjing General Hospital of Nanjing Command, PLIA, Nanjing, Jiangsu 210002, China.
Zhonghua Nan Ke Xue. 2006 Aug;12(8):703-5.
To evaluate the correlation of total antioxidant capacity (TAC) in seminal plasma with sperm motility in infertile men, and explore the clinical significance of TAC in seminal plasma in male fertility.
One hundred and thirteen infertile men with normal sperm density were included in the experiment group and 28 fertile men in the normal control. The seminal parameter analysis was performed by computer-assisted semen analysis (CASA) system. Seminal plasma TAC was measured with spectroscopic analysis.
Seminal plasma TAC was (14.37 +/- 8.45) U in the infertile men with normal sperm density and (19.82 +/- 6.33) U in the fertile control. Compared with the fertile men, seminal plasma TAC in the experiment group was significantly lower (P < 0.01). There was significant correlation between seminal plasma TAC and sperm motility, grade a sperm (r = 0.208, P < 0.05), grade (a + b) sperm (r = 0.231, P < 0.05), straightness (STR) (r = 0.200, P < 0.05), linearity (LIN) (r = 0.208, P < 0.05), curvilinear velocity (VCL) (r = 0.189, P < 0.05), straight line velocity (VSL) (r = 0.210, P < 0.05), average path velocity (VAP) (r = 0.215, P < 0.05), and beat cross frequency (BCF) (r = -0.248, P < 0.01). There was no significant correlation among the average motion degree (MAD), the amplitude of lateral head displacement (ALH) and wobbly (WOB).
TAC in seminal plasma is closely related to male fertility, appropriate TAC provides a favourable environment for sperm swimming. The decreased level of TAC in seminal plasma may be one of the causes of male infertility. The analysis of TAC in seminal plasma may afford valuable evidence in exploring the mechanism of male infertility and in clinical medication.
评估不育男性精浆中总抗氧化能力（TAC）与精子活力的相关性，探讨精浆TAC在男性生育中的临床意义。
实验组纳入113例精子密度正常的不育男性，正常对照组纳入28例生育男性。采用计算机辅助精液分析（CASA）系统进行精液参数分析。用光谱分析法测定精浆TAC。
精子密度正常的不育男性精浆TAC为（14.37±8.45）U，生育对照组为（19.82±6.33）U。与生育男性相比，实验组精浆TAC显著降低（P<0.01）。精浆TAC与精子活力、a级精子（r=0.208，P<0.05）、（a+b）级精子（r=0.231，P<0.05）、直线性（STR）（r=0.200，P<0.05）、线性度（LIN）（r=0.208，P<0.05）、曲线速度（VCL）（r=0.189，P<0.05）、直线速度（VSL）（r=0.210，P<0.05）、平均路径速度（VAP）（r=0.215，P<0.05）及鞭打频率（BCF）（r=-0.248，P<0.01）之间存在显著相关性。平均运动度（MAD）、头部侧摆幅度（ALH）及摆动性（WOB）之间无显著相关性。
精浆TAC与男性生育密切相关，适当的TAC为精子游动提供有利环境。精浆TAC水平降低可能是男性不育的原因之一。精浆TAC分析可为探讨男性不育机制及临床用药提供有价值的依据。
