Liu X Y, Yu J L, Zheng H D, Chen Y, Shen J F, Feng Z H, Chen Z C, You L F, Sun B H, Zhou Y Y
Shanghai Institute of Biochemistry, Chinese Academy of Sciences.
Chin J Biotechnol. 1991;7(1):25-31.
Recombinant interleukin-2 (rIL-2) is able to maintain the growth and multiplication of T-lymphocytes and IL-2 dependent CTLL-2 cells up to 28 days with the multiplication of T-lymphocytes as high as about 1000 fold. When rIL-2 and its monoclonal antibody were added together, the above functions of rIL-2 were specially blocked, showing that these biological functions were exerted specifically by rIL-2. The rIL-2 is also able to increase the natural killer (NK) activity up to 46-96%, while higher concentrations of rIL-2 might exhibit inhibitory effect. The cytotoxicity of lymphokine activated killer (LAK) cells induced by rIL-2 for killing HL-60 cell line and solid tumour (human lung carcinoma) reached 54.84% and 37.96%, respectively. All these results indicate that the biological functions of rIL-2 were quite similar to that of the natural one.
重组白细胞介素-2（rIL-2）能够维持T淋巴细胞和IL-2依赖的CTLL-2细胞的生长和增殖长达28天，T淋巴细胞的增殖高达约1000倍。当rIL-2与其单克隆抗体一起添加时，rIL-2的上述功能被特异性阻断，表明这些生物学功能是由rIL-2特异性发挥的。rIL-2还能够将自然杀伤（NK）活性提高46%-96%，而更高浓度的rIL-2可能表现出抑制作用。rIL-2诱导的淋巴因子激活的杀伤（LAK）细胞对HL-60细胞系和实体瘤（人肺癌）的细胞毒性分别达到54.84%和37.96%。所有这些结果表明，rIL-2的生物学功能与天然白细胞介素-2非常相似。
