肾上腺肿瘤细胞培养中的类固醇生成与细胞外环磷酸腺苷（cAMP）积累

Steroidogenesis and extracellular cAMP accumulation in adrenal tumor cell cultures.

作者信息

Schimmer B P, Zimmerman A E

出版信息

Mol Cell Endocrinol. 1976 Mar;4(4):263-70. doi: 10.1016/0303-7207(76)90060-5.

Abstract

ACTH stimulated steroidogenesis and cAMP (adenosine 3',5'-monophosphate) accumulation in an adrenocortical mouse tumor cell line (clone Y1) with Kd values which differed by more than one order of magnitude (5.2 X 10(-11) M and 7 X 10(-10) M, respectively). All of the cAMP formed in response to added ACTH appeared extracellularly in 5- or 30-min incubations. ACTH, at 5 and 10 muU/ml, stimulated steroidogenesis to 25% and 40% of maximum activity; and increased the extracellular accumulation of cAMP 1.4-fold and 2.3-fold, respectively. The effects of ACTH appeared to be via an action on intracellular ATP, specific for cAMP and dependent on an ACTH-sensitive adenylate cyclase system. These observations indicate that ACTH increases cAMP accumulation in Y1 cells at virtually all steroidogenic concentrations and suggest that cAMP is an essential component of ACTH-stimulated steroidogenesis.

摘要

促肾上腺皮质激素（ACTH）刺激肾上腺皮质小鼠肿瘤细胞系（克隆Y1）中的类固醇生成和环磷酸腺苷（cAMP）积累，其解离常数（Kd值）相差一个多数量级（分别为5.2×10⁻¹¹ M和7×10⁻¹⁰ M）。在5分钟或30分钟的孵育中，所有因添加ACTH而形成的cAMP都出现在细胞外。5和10微单位/毫升的ACTH将类固醇生成刺激至最大活性的25%和40%；并分别使cAMP的细胞外积累增加1.4倍和2.3倍。ACTH的作用似乎是通过对细胞内ATP的作用，这种作用对cAMP具有特异性，并依赖于ACTH敏感的腺苷酸环化酶系统。这些观察结果表明，在几乎所有类固醇生成浓度下，ACTH都会增加Y1细胞中cAMP的积累，并表明cAMP是ACTH刺激类固醇生成的重要组成部分。