Kimura K, Kamiyama Y, Ozawa K, Honjo I
Gastroenterology. 1976 May;70(5 PT.1):665-8.
Changes in the adenine nucleotide metabolism after an oral glucose load were studied in the liver of normal and alloxan-diabetic rats. Changes in the energy charge were positively correlated with those in the blood glucose and plasma immunoreactive insulin levels. One hour after an oral glucose load when the plasma immunoreactive insulin levels increased maximally, the energy charge increased maximally from 0.846 to 0.867 (P less than 0.001). The increase in the energy charge was accompanied by a concomitant decrease in the ADP levels (P less than 0.05). The respiratory control ration, state 3 respiration per unit of cytochrome a (+a3), and DNP-induced ATPase activity per unit of cytochrome a (+a3) increased significantly. The adenylate kinase and pyruvate kinase activities in the liver remained unchanged. On the other hand, the energy charge in the liver of alloxan-diabetic rats did not increase significantly after an oral glucose load. It was suggested that an increase in the energy charge of the liver is attributable to the more rapid flux of intermediary metabolism in the enhanced ADP-phosphorylating reactions by mitochondria, owing to an elevated level of insulin available to the hepatic cells.
在正常大鼠和四氧嘧啶糖尿病大鼠的肝脏中，研究了口服葡萄糖负荷后腺嘌呤核苷酸代谢的变化。能荷的变化与血糖及血浆免疫反应性胰岛素水平的变化呈正相关。口服葡萄糖负荷1小时后，当血浆免疫反应性胰岛素水平达到最大值时，能荷从0.846最大增加到0.867（P<0.001）。能荷的增加伴随着ADP水平的相应降低（P<0.05）。呼吸控制率、每单位细胞色素a（+a3）的状态3呼吸以及每单位细胞色素a（+a3）的二硝基苯酚诱导的ATP酶活性显著增加。肝脏中的腺苷酸激酶和丙酮酸激酶活性保持不变。另一方面，口服葡萄糖负荷后，四氧嘧啶糖尿病大鼠肝脏中的能荷没有显著增加。提示肝脏能荷的增加归因于肝细胞可利用的胰岛素水平升高，使得线粒体中ADP磷酸化反应增强，中间代谢通量加快。
