Verschueren H, De Baetselier P, Bereiter-Hahn J
Pasteur Instituut van Brabant, Brussels, Belgium.
Cell Motil Cytoskeleton. 1991;20(3):203-14. doi: 10.1002/cm.970200304.
We have used an in vitro model system to analyze cytomechanical aspects of tissue infiltration by T-lymphocytes. The interaction of metastatic T-lymphoma cells with a precultured monolayer of 10T1/2 fibroblast-like cells was recorded in time-lapse video with alternating phase contrast and reflection interference contrast microscopy. Sectioning of embedded specimens as well as cytoskeletal stainings have been performed on matching cocultures. The lymphoma cells did not strongly attach or spread on the dorsal surface of the monolayer cells. Invasion started with the protrusion of a pseudopodium through a narrow gap, and conspicuous constriction of the invading cell's body and nucleus was a consistent feature during the later steps. Overt retraction of the target cells was not seen, but the invading lymphoma cells elevated the fibroblasts over relatively large areas, thereby creating dome-shaped open spaces, allowing for further migration under the monolayer with minimal resistance. Invasion was not unidirectional but was readily reversible at any stage. Due to this wavering character, an invasion event could take more than 1 hour, although the shape alterations involved were fast. Even after the invasion process had been completed, the lymphoma cells could come out from below the monolayer again. Therefore we propose that invasion in this model should be considered as a dynamic equilibrium. Invading T-lymphoma cells displayed diffuse F-actin staining and a well-organized microtubular complex with the centrosomes behind the nucleus in the uropod, which also contained most vesicular organelles.
我们使用了一种体外模型系统来分析T淋巴细胞对组织浸润的细胞力学方面。通过交替相衬和反射干涉对比显微镜的延时视频记录转移性T淋巴瘤细胞与预培养的10T1/2成纤维样细胞单层的相互作用。对匹配的共培养物进行了包埋标本切片以及细胞骨架染色。淋巴瘤细胞在单层细胞的背表面上没有强烈附着或扩散。侵袭开始于伪足通过狭窄间隙突出，并且在后期步骤中，侵袭细胞的主体和细胞核明显收缩是一个一致的特征。未观察到靶细胞明显回缩，但侵袭性淋巴瘤细胞在相对较大的区域上抬高了成纤维细胞，从而形成圆顶状的开放空间，允许在单层下以最小阻力进一步迁移。侵袭不是单向的，而是在任何阶段都很容易逆转。由于这种摇摆特性，尽管涉及的形状改变很快，但侵袭事件可能需要超过1小时。即使在侵袭过程完成后，淋巴瘤细胞也可以再次从单层下方出来。因此，我们建议在该模型中的侵袭应被视为一种动态平衡。侵袭性T淋巴瘤细胞显示出弥漫性F-肌动蛋白染色和一个组织良好的微管复合体，其中心体位于尾足中细胞核的后方，尾足中也含有大多数囊泡细胞器。
