Verschueren H, Dewit J, De Braekeleer J, Schirrmacher V, De Baetselier P
Pasteur Instituut van Brabant, Brussels, Belgium.
Cell Biol Int. 1994 Jan;18(1):11-9. doi: 10.1006/cbir.1994.1002.
ESb and BW-O-Li1 are T-lymphoma cell lines that form metastases in various organs after injection into syngeneic mice. In vitro, both cell lines invade through a fibroblastic monolayer, but ESb cells do so much slower than BW-O-Li1. By the use of Fourier analysis of cell outlines, we can relate this difference in invasiveness to a difference in cell motility: ESb cells do not perform any conspicuous shape change, whereas BW-O-Li1 cells are actively protruding and retracting large pseudopodia. However, the low-motile ESb cells become as motile and deformable as BW-O-Li1 cells when they have eventually invaded under a fibroblastic monolayer. This indicates that ESb cells do have inherent capability for shape change. Treatment of ESb cells with the microtubule disrupting agent nocodazole concomitantly increases their shape change intensity, and their invasion rate through fibroblast monolayers. On the contrary, the microtubule stabilizing drug taxol inhibits both motility and invasion of BW-O-Li1 cells. Our observations suggest that the microtubule network can repress invasion-bound motility of lymphoid cells.
ESb和BW-O-Li1是T淋巴瘤细胞系，将其注射到同基因小鼠体内后会在各个器官形成转移灶。在体外，这两种细胞系都能穿过成纤维细胞单层，但ESb细胞的侵袭速度比BW-O-Li1细胞慢得多。通过对细胞轮廓进行傅里叶分析，我们可以将这种侵袭性差异与细胞运动性差异联系起来：ESb细胞没有明显的形状变化，而BW-O-Li1细胞则积极地伸出和缩回大的伪足。然而，低运动性的ESb细胞最终在成纤维细胞单层下侵袭时，会变得与BW-O-Li1细胞一样具有运动性和可变形性。这表明ESb细胞确实具有形状变化的内在能力。用微管破坏剂诺考达唑处理ESb细胞会同时增加其形状变化强度以及它们穿过成纤维细胞单层的侵袭率。相反，微管稳定剂紫杉醇会抑制BW-O-Li1细胞的运动性和侵袭。我们的观察结果表明，微管网络可以抑制淋巴细胞与侵袭相关的运动性。
