Guo R, Lim C K
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
J Chromatogr. 1991 Jul 26;550(1-2):603-7. doi: 10.1016/s0021-9673(01)88566-6.
A reversed-phase high-performance liquid chromatographic method is described for the determination of hydroxy and peroxy acid derivatives of uroporphyrin in the plasma of patients with congenital erythropoietic porphyria. The porphyrins were extracted from the plasma with 20% trichloroacetic acid-dimethyl sulphoxide (1:1, v/v). The supernatant after centrifugation was chromatographed on a Hypersil-ODS column by gradient elution with 9% (v/v) acetonitrile in 1 M ammonium acetate buffer (pH 5.16) (solvent A) and 10% (v/v) acetonitrile in methanol (solvent B) as the gradient mixture. The method was also suitable for the preparative isolation of the porphyrins.
描述了一种反相高效液相色谱法，用于测定先天性红细胞生成性卟啉症患者血浆中尿卟啉的羟基和过氧酸衍生物。用20%三氯乙酸 - 二甲基亚砜（1:1，v/v）从血浆中提取卟啉。离心后的上清液在Hypersil-ODS柱上进行色谱分离，以1 M醋酸铵缓冲液（pH 5.16）中9%（v/v）乙腈（溶剂A）和甲醇中10%（v/v）乙腈（溶剂B）作为梯度混合物进行梯度洗脱。该方法也适用于卟啉的制备性分离。
