Kikukawa Y, Ishikawa M, Sengoku K, Kasamo M, Shimizu T
Department of Obstetrics and Gynecology, Asahikawa Medical College, Japan.
Prostaglandins. 1991 Aug;42(2):95-104. doi: 10.1016/0090-6980(91)90069-r.
The mechanism of ovulation has been compared to an inflammatory reaction. Platelet activating factor (PAF) is an important mediator of inflammation as it may induce the production of prostaglandins and lysosomal enzyme. We evaluated the potential role of PAF in PMSG-HCG induced ovulation using CV3988, a specific PAF receptor antagonist in a superovulated ICR mice (9-12 weeks old). CV3988 blocked the ovulation in a dose dependent manner, and the significant reduced ovulatory efficiency was observed at more than 500 micrograms dose (p less than 0.001). The ovulatory efficiency reduced by CV3988 was reversed by PAF in a dose dependent manner. In vitro fertilization (IVF) rate of follicular oocytes with treatment of CV3988 was not different from that of ovulated ova without treatment. These results suggest that PAF may be involved in the ovulation process but the presence of PAF may not be essential for the fertilization of the ova as IVF.
排卵机制已被比作一种炎症反应。血小板活化因子（PAF）是炎症的重要介质，因为它可能诱导前列腺素和溶酶体酶的产生。我们使用CV3988（一种超排卵的ICR小鼠（9 - 12周龄）中的特异性PAF受体拮抗剂）评估了PAF在孕马血清促性腺激素 - 人绒毛膜促性腺激素（PMSG - HCG）诱导排卵中的潜在作用。CV3988以剂量依赖性方式阻断排卵，在超过500微克剂量时观察到排卵效率显著降低（p小于0.001）。PAF以剂量依赖性方式逆转了CV3988降低的排卵效率。用CV3988处理的卵泡卵母细胞的体外受精（IVF）率与未处理的排卵卵的IVF率没有差异。这些结果表明，PAF可能参与排卵过程，但PAF的存在对于卵母细胞作为IVF的受精可能不是必需的。
