Ji S, Hyun J, Park E, Lee B-L, Kim K-K, Choi Y
Program of Oromaxillofacial Infection & Immunity and BK21, School of Dentistry, Seoul National University, Seoul, Korea.
J Periodontal Res. 2007 Oct;42(5):410-9. doi: 10.1111/j.1600-0765.2006.00962.x.
The aim of this study was to compare the susceptibility of nonperiodontopathic and periodontopathic bacteria to major defense mechanisms for bacterial clearance in gingival sulcus.
Twenty strains of 13 oral bacterial species were studied for their susceptibility to phagocytosis by human neutrophils and to the antimicrobial peptides LL-37 and human beta defensin-3. The minimum inhibitory concentrations of LL-37 and human beta defensin-3 were determined by a liquid dilution assay, and susceptibility to phagocytosis was examined by a flow cytometric phagocytosis assay.
The minimum inhibitory concentrations of LL-37 and human beta defensin-3 varied greatly, depending on the strain and species. Although a significant difference between the non- and periodontopathic groups was not observed, the red-complex bacteria were more resistant to LL-37 than the others (p=0.004). The susceptibility of oral bacteria to phagocytosis was quite variable, depending on the species but not on the strains. The periodontopathic bacteria, especially Actinobacillus actinomycetemcomitans and the red-complex triad, were more resistant to phagocytosis than were the nonperiodontopathic bacteria (p=0.0003). In addition, bacteria resistant both to antimicrobial peptides and to phagocytosis were more common in the periodontopathic group.
Our results indicate that immune evasion may contribute to the pathogenicity of some periodontopathic bacteria.
本研究旨在比较非牙周病相关细菌和牙周病相关细菌对龈沟内细菌清除主要防御机制的敏感性。
研究了13种口腔细菌的20株菌株对人中性粒细胞吞噬作用以及抗菌肽LL-37和人β-防御素-3的敏感性。通过液体稀释法测定LL-37和人β-防御素-3的最低抑菌浓度，并通过流式细胞术吞噬试验检测吞噬敏感性。
LL-37和人β-防御素-3的最低抑菌浓度因菌株和菌种而异。虽然在非牙周病组和牙周病组之间未观察到显著差异，但红色复合体细菌比其他细菌对LL-37更具抗性（p=0.004）。口腔细菌对吞噬作用的敏感性差异很大，取决于菌种而非菌株。牙周病相关细菌，尤其是伴放线放线杆菌和红色复合体三联菌，比非牙周病相关细菌对吞噬作用更具抗性（p=0.0003）。此外，对抗菌肽和吞噬作用均具有抗性的细菌在牙周病组中更为常见。
我们的结果表明，免疫逃逸可能有助于某些牙周病相关细菌的致病性。
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