使用棋盘法对含精油口腔条对唾液细菌的影响进行体内评估。

da Silva Carina Maciel, Colombo Andréa Vieira, do Souto Renata Martins, Colombo Ana Paula

Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

J Clin Dent. 2005;16(2):38-43.

OBJECTIVE

The aim of this study was to evaluate the antimicrobial effect of essential oil-containing oral strips on different species of the oral microbiota.

METHODOLOGY

Saliva samples were collected from 20 subjects with good oral health, diluted and plated onto blood agar medium. The subjects were asked to place the strip (Listerine PocketPaks) on the tongue allowing it to dissolve. After 30 minutes, new saliva samples were collected again and the plates with the samples were incubated under anaerobic conditions at 37 degrees C for seven days. Colony counts (CFU/mL) were determined for each sample. The colonies on the plates were washed with 1 mL of TE buffer, and the bacterial suspensions were processed for the identification of 24 species by DNA probes and the Checkerboard DNA-DNA hybridization method. Differences in total counts, prevalence, and levels of the species evaluated before and after placement of the strips were determined by Wilcoxon sign rank and Chi-square tests.

RESULTS

A modest increase in the total bacterial number in saliva from 1.4 x 10(8) to 1.7 x 10(8) bacterial cells was observed 30 minutes after the strip placement, although this change was not significant (p = 0.632). Most of the species reduced in frequency and/or levels, including the pathogens A. actinomycetemcomitans, C. rectus, E. corrodens, Fusobacterium spp., P. intermedia, and S. noxia, as well as the beneficial species A. meyeri, A. georgia, A. gerencseriae, A. odontolyticus, and P. acnes after strip placement. In contrast, A. viscosus, P. melaninogenica, P. gingivalis, P. micros, Streptococcus spp., T. forsythensis, and V. parvula presented an increase in prevalence and/or levels. These changes were not statistically significant after adjusting for multiple comparisons (p > 0.0022).

CONCLUSION

The use of the essential oil-containing oral strips resulted in a short-term small increase in the total number of salivary microorganisms. In addition, a not significant decrease of certain periodontopathogens, and an increase in species compatible with oral health were observed.

目的

本研究旨在评估含精油口腔条对口腔微生物群不同物种的抗菌效果。

方法

从20名口腔健康状况良好的受试者收集唾液样本，稀释后接种于血琼脂培养基上。要求受试者将口腔条（李斯德林便携式漱口水）置于舌上使其溶解。30分钟后，再次收集新的唾液样本，并将装有样本的培养皿在37℃厌氧条件下培养7天。测定每个样本的菌落计数（CFU/mL）。用1 mL TE缓冲液冲洗培养皿上的菌落，并用DNA探针和棋盘式DNA-DNA杂交法对细菌悬液进行处理，以鉴定24种细菌。通过Wilcoxon符号秩检验和卡方检验确定放置口腔条前后所评估物种的总数、流行率和水平的差异。

结果

放置口腔条30分钟后，观察到唾液中细菌总数略有增加，从1.4×10⁸个细菌细胞增加到1.7×10⁸个细菌细胞，尽管这一变化不显著（p = 0.632）。放置口腔条后多数物种的频率和/或水平降低，包括病原菌伴放线放线杆菌、直肠弯曲菌、啮蚀艾肯菌、梭杆菌属、中间普氏菌和具核梭杆菌，以及有益菌迈耶放线菌、佐治亚放线菌、杰氏放线菌、溶牙放线菌和痤疮丙酸杆菌。相反，黏性放线菌、产黑色素普氏菌、牙龈卟啉单胞菌、微小消化链球菌、链球菌属、福赛坦氏菌和小韦荣球菌的流行率和/或水平有所增加。在进行多重比较校正后，这些变化无统计学意义（p > 0.0022）。