Wang Jun, Li Biao, Cai Changping, Zhang Yifan, Wang Shili, Hu Shengping, Tian Xianglong, Zhang Min
Department of Otolaryngology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Neuroreport. 2007 Aug 27;18(13):1329-33. doi: 10.1097/WNR.0b013e3282010b16.
Degeneration of peripheral auditory neurons constitutes one of the main causes of sensorineural hearing loss. Gene delivery to the inner ear is central to the development of gene therapy for hearing impairment. Thus we investigated the effectiveness of baculovirus-derived vectors to transduce spiral ganglion neurons. We found that baculovirus could efficiently transduce spiral ganglion neurons in vitro and that the highest transduced cell rate could be over 75%. The level of transgene expression exhibited viral dose dependence and was enhanced by the addition of butyrate. Thus, baculovirus is a novel and promising tool for gene transfer into the cochlear nervous system, both in studies of the function of foreign genes and in the development of gene-therapy strategies.
外周听觉神经元的退化是感音神经性听力损失的主要原因之一。向内耳进行基因传递是听力障碍基因治疗发展的核心。因此，我们研究了杆状病毒衍生载体转导螺旋神经节神经元的有效性。我们发现杆状病毒能够在体外有效地转导螺旋神经节神经元，最高转导细胞率可达75%以上。转基因表达水平呈现病毒剂量依赖性，并通过添加丁酸盐而增强。因此，杆状病毒是一种新型且有前景的工具，可用于将基因导入耳蜗神经系统，无论是在外源基因功能研究还是基因治疗策略的开发中。
