Kodama Satoru, Hirano Takashi, Abe Nobuyuki, Suzuki Masashi
Department of Otolaryngology, Oita University Faculty of Medicine, Oita, Japan.
Acta Otolaryngol. 2007 Aug;127(8):809-15. doi: 10.1080/00016480601075365.
Nasal vaccination with P6 protein of nontypeable Haemophilus influenzae (NTHi) and CpG oligodeoxynucleotide (ODN) may be effective for the induction of NTHi-specific protective immunity in the middle ear and middle ear application of CpG ODN may possibly be a new treatment strategy for otitis media (OM).
Owing to the increased prevalence of antibiotic-resistant bacteria in recent years, treatment for OM often fails. In the interest of developing an alternative treatment that does not require antibiotics, the efficacy of middle ear application of CpG ODN was examined.
Mice were immunized intranasally with P6 and CpG ODN, and CpG ODN was then inoculated into the middle ear. P6-specific antibody titers were determined by enzyme-linked immunosorbent assay (ELISA) and the numbers of P6-specific antibody-producing cells were determined by enzyme-linked immunospot (ELISPOT) assay.
P6-specific IgA in ear wash and serum IgG titers were elevated after nasal immunization. The number of specific IgA-producing cells was markedly increased in the middle ear mucosa. Interestingly, middle ear application of CpG ODN enhanced IgA levels in the middle ear.
用不可分型流感嗜血杆菌（NTHi）的P6蛋白和CpG寡脱氧核苷酸（ODN）进行鼻腔接种可能有效诱导中耳产生NTHi特异性保护性免疫，且在中耳应用CpG ODN可能是中耳炎（OM）的一种新治疗策略。
由于近年来抗生素耐药菌的患病率增加，OM的治疗常常失败。为了开发一种不需要抗生素的替代治疗方法，研究了在中耳应用CpG ODN的疗效。
用P6和CpG ODN对小鼠进行鼻腔免疫，然后将CpG ODN接种到中耳。通过酶联免疫吸附测定（ELISA）测定P6特异性抗体滴度，通过酶联免疫斑点（ELISPOT）测定法测定产生P6特异性抗体的细胞数量。
鼻腔免疫后，耳洗液中的P6特异性IgA和血清IgG滴度升高。中耳黏膜中产生特异性IgA的细胞数量显著增加。有趣的是，在中耳应用CpG ODN可提高中耳中的IgA水平。
