验证的液相色谱法用于定量测定蒜粉和片剂中的大蒜素。

Validated liquid chromatographic method for quantitative determination of allicin in garlic powder and tablets.

作者信息

de Diego Marta, Avello Marcia, Mennickent Sigrid, Fernández Marcos, Fernández Pola

机构信息

Pharmacy Department, Pharmacy School, University of Concepción, Concepción, Chile.

出版信息

J Sep Sci. 2007 Nov;30(16):2703-7. doi: 10.1002/jssc.200700161.

DOI:10.1002/jssc.200700161

PMID:17763522

Abstract

In the present study, an RP high performance liquid chromatographic method was developed and validated for the determination of allicin in garlic powder and tablets. Chromatographic separation was carried out on an RP-18(e )column (125 mm x 4 mm), using a mobile phase, consisting of methanol-water (50:50 v/v), at a flow rate of 0.5 mL/min and UV detection at 220 nm. Ethylparaben was used as the internal standard. The assay was linear for allicin concentrations of 5.0-60.0 microg/mL. The RSD for precision was <6.14%. The accuracy was above 89.11%. The detection and quantification limits were 0.27 and 0.81 microg/mL, respectively. This method was used to quantify allicin in garlic powder samples. The results showed that the method described here is useful for the determination of allicin in garlic powder and tablets.

摘要

在本研究中，开发并验证了一种反相高效液相色谱法，用于测定蒜粉和片剂中的大蒜素。色谱分离在反相-18(e)柱（125 mm×4 mm）上进行，流动相为甲醇-水（50:50 v/v），流速为0.5 mL/min，紫外检测波长为220 nm。对羟基苯甲酸乙酯用作内标。该测定法在大蒜素浓度为5.0 - 60.0 μg/mL范围内呈线性。精密度的相对标准偏差小于6.14%。准确度高于89.11%。检测限和定量限分别为0.27和0.81 μg/mL。该方法用于定量蒜粉样品中的大蒜素。结果表明，本文所述方法可用于测定蒜粉和片剂中的大蒜素。