Tang Qingfa, Yang Chunhua, Ye Wencai, Liu Jinghan, Zhao Shouxun
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, People's Republic of China.
Phytochem Anal. 2008 Mar-Apr;19(2):155-9. doi: 10.1002/pca.1030.
Aconitum coreanum (Lèvl.) Rapaics (Guanbaifu in Chinese) is a widely used, centuries-old Chinese herb. A preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) method was employed for isolation and purification of alkaloids from the crude extract of Aconitum coreanum (Lèvl.) Rapaics using ethyl acetate-n-butanol-methanol-0.2 m HCl (7:2:2:7, v/v) as a two-phase solvent system. Six alkaloids, including GFO, GFQ, GFZ, hetisinone, hetisine and GFAA, were obtained in one-step separation. The purity of these compounds was 97.6, 93.8, 91.8, 91.9, 96.2 and 91.1%, respectively.
朝鲜乌头（Aconitum coreanum (Lèvl.) Rapaics，中文名为关白附）是一种广泛使用的、有着数百年历史的中药材。采用制备型高速逆流色谱（HSCCC）结合蒸发光散射检测（ELSD）方法，以乙酸乙酯 - 正丁醇 - 甲醇 - 0.2 m盐酸（7:2:2:7，v/v）作为两相溶剂体系，从朝鲜乌头（Aconitum coreanum (Lèvl.) Rapaics）粗提物中分离纯化生物碱。一步分离得到了6种生物碱，包括GFO、GFQ、GFZ、异叶乌头碱、乌头碱和GFAA。这些化合物的纯度分别为97.6%、93.8%、91.8%、91.9%、96.2%和91.1%。
