Haley Jeffrey C, Liu Yuanqin, Winnik Mitchell A, Demmer David, Haslett Tom, Lau Willie
Department of Chemistry, University of Toronto, Toronto, Ontario, Canada.
Rev Sci Instrum. 2007 Aug;78(8):084101. doi: 10.1063/1.2766844.
We describe an instrument to measure the polymer interdiffusion between donor-labeled and acceptor-labeled latex polymers in a partially wet latex film with fluorescence resonance energy transfer (FRET). It is possible to temporarily arrest the drying process of a wet latex film by sealing the film in an airtight chamber. In our approach, we measure donor fluorescence decays from 0.5 mm diameter spots at various positions across an arrested latex film with time-correlated single photon counting. We interpret the resulting decays with a Monte Carlo simulation of the FRET process and extract information about the extent of polymer diffusion as a function of position on the film. These results enable us to determine the extent of polymer interdiffusion as a function of distance from the wet-dry edge in the latex film. To highlight this device's ability to capture the rapid early stages of latex interdiffusion, we report results from an acrylate copolymer latex.
我们描述了一种利用荧光共振能量转移（FRET）来测量部分湿润乳胶膜中供体标记和受体标记的乳胶聚合物之间聚合物相互扩散的仪器。通过将乳胶膜密封在气密室内，可以暂时阻止湿润乳胶膜的干燥过程。在我们的方法中，我们使用时间相关单光子计数法测量在被阻止干燥的乳胶膜上不同位置直径为0.5毫米的斑点处供体荧光随时间的衰减。我们用FRET过程的蒙特卡罗模拟来解释所得的衰减，并提取有关聚合物扩散程度随膜上位置变化的信息。这些结果使我们能够确定聚合物相互扩散程度随乳胶膜中干湿边缘距离的变化情况。为了突出该装置捕捉乳胶相互扩散快速早期阶段的能力，我们报告了丙烯酸酯共聚物乳胶的实验结果。
