Valentine Tracy A, Randall Eva, Wypijewski Krzysztof, Chapman Sean, Jones John, Oparka Karl J
Scottish Crop Research Institute, Invergowrie, Dundee, Scotland DD2 5DA, UK.
Plant Biotechnol J. 2007 Nov;5(6):827-34. doi: 10.1111/j.1467-7652.2007.00289.x. Epub 2007 Aug 31.
Plant parasitic nematodes cause significant damage to crops on a worldwide scale. These nematodes are often soil dwelling but rely on plants for food and to sustain them during reproduction. Complex interactions occur between plants and nematodes during the nematode life cycle with plant roots developing specialized feeding structures through which nematodes withdraw nutrients. Here we describe a novel method for delivering macromolecules to feeding nematodes using a virus-based vector [tobacco rattle virus (TRV)]. We show that the parasitic nematode Heterodera schachtii will ingest fluorescent proteins transiently expressed in plant roots infected with a TRV construct carrying the appropriate protein sequence. A prerequisite for this delivery is the presence of replicating virus in root tips prior to the formation of nematode-induced syncytia. We show also that TRV vectors expressing nematode gene sequences can be used to induce RNAi in the feeding nematodes.
植物寄生线虫在全球范围内对农作物造成严重损害。这些线虫通常生活在土壤中，但依靠植物获取食物并在繁殖过程中维持生存。在 nematode 生命周期中，植物与线虫之间会发生复杂的相互作用，植物根会发育出专门的取食结构，线虫通过这些结构摄取营养。在这里，我们描述了一种使用基于病毒的载体[烟草脆裂病毒（TRV）]将大分子递送至取食线虫的新方法。我们表明，寄生线虫甜菜胞囊线虫会摄取在感染携带适当蛋白质序列的TRV构建体的植物根中瞬时表达的荧光蛋白。这种递送的一个先决条件是在形成线虫诱导的多核细胞之前根尖中存在复制病毒。我们还表明，表达线虫基因序列的TRV载体可用于在取食线虫中诱导RNA干扰。
