Karanastasi E, Wyss U, Brown D
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2001;66(2b):599-608.
Feeding by P. anemones, an efficient vector of tobacco rattle virus (TRV), was investigated by video-enhanced interference light microscopy. Four stages were observed after transfer of individual nematodes, extracted from soil, to Nicotiana tabacum seedling roots in agar: i) acclimatisation; ii) approach and scrutiny; iii) preparation; and iv) feeding. Prior to commencement of stage 'iv' about 4 cells perforated by rapid onchiostyle thrusting remained alive, each having been almost immediately abandoned by the nematode. During the stage iv) approximately 5% of perforated cells remained alive. Feeding on individual cells was similar to that previously reported for Trichodorus similis: cells from which cytoplasm was ingested after a prolonged period of salivation were invariably killed, with adjacent cells being unaffected. During feeding a number of cells perforated but soon afterwards abandoned by P. anemones remained alive, providing an effective pathway for successful transmission of TRV to plants by the nematode.
通过视频增强干涉光显微镜研究了作为烟草脆裂病毒（TRV）有效传播媒介的毛刺线虫的取食情况。从土壤中提取的单个线虫转移到琼脂中的烟草幼苗根部后，观察到四个阶段：i）适应阶段；ii）接近与审视阶段；iii）准备阶段；以及iv）取食阶段。在“iv”阶段开始之前，大约有4个被快速的口针穿刺穿孔的细胞仍然存活，每个细胞几乎立即被线虫抛弃。在阶段iv）期间，大约5%的穿孔细胞仍然存活。对单个细胞的取食情况与先前报道的相似瘤线虫类似：经过长时间唾液分泌后细胞质被摄取的细胞总是会死亡，相邻细胞则不受影响。在取食过程中，许多被毛刺线虫穿刺但随后很快被抛弃的细胞仍然存活，为线虫将TRV成功传播到植物提供了一条有效途径。
