Carelli Donatella, Centonze Diego, Palermo Carmen, Quinto Maurizio, Rotunno Taddeo
DiSACD-Dipartimento di Scienze Agro-Ambientali, Chimica e Difesa Vegetale and BIOAGROMED, Università degli Studi di Foggia, via Napoli, 25-71100, Foggia, Italy.
Biosens Bioelectron. 2007 Dec 15;23(5):640-7. doi: 10.1016/j.bios.2007.07.008. Epub 2007 Jul 28.
In this work is reported the development and application of an amperometric biosensor for the determination of total biogenic amines content by using the commercial diamino oxidase (DAO from Porcine kidney E.C. 1.4.3.6) as the biocomponent, entrapped by glutaraldehyde onto an electrosynthesized bilayer film. In order to minimize both the fouling and the interference caused by the direct electrochemical oxidation of both the analytes (i.e., biogenic amines) and the common interferents usually present in food products the performances of Pt and Au electrodes and of several electroproduced anti-interferents mono- and bi-layer films were tested. In spite of a very low activity of the commercial DAO, the biosensor displayed a high response sensitivity in flow experiments, short response time, a good linear response and low detection limits. The excellent anti-interference characteristics allowed the use of the biosensor in screening analysis of food products.
本文报道了一种安培型生物传感器的研制与应用，该传感器以商业二胺氧化酶（猪肾来源的E.C. 1.4.3.6二胺氧化酶）作为生物组分，通过戊二醛固定在电合成双层膜上，用于测定总生物胺含量。为了尽量减少分析物（即生物胺）和食品中常见干扰物直接电化学氧化所引起的污染和干扰，测试了铂电极和金电极以及几种电合成抗干扰单层和双层膜的性能。尽管商业二胺氧化酶活性很低，但该生物传感器在流动实验中表现出高响应灵敏度、短响应时间、良好的线性响应和低检测限。其优异的抗干扰特性使得该生物传感器可用于食品的筛选分析。
