脊髓灰质炎病毒的沉降离心。

SEDIMENTATION OF POLIOMYELITIS VIRUS BY CENTRIFUGATION.

出版信息

Science. 1945 Oct 12;102(2650):380-2. doi: 10.1126/science.102.2650.380.

DOI:10.1126/science.102.2650.380

Abstract

A technic is described whereby poliomyelitis virus may be sedimented by centrifugation for four hours at 18,000 r.p.m. Virus has been recovered quantitatively in the sediment from a 20 per cent., but not a 1 per cent., saline suspension. The addition of 10 per cent. normal serum results in quantitative recovery from a 1 per cent. suspension. Virus has been recovered by intracerebral inoculation from two of four human stools tested by the method described.

摘要

描述了一种技术，通过该技术，脊髓灰质炎病毒可以在 18,000 r.p.m. 的转速下离心沉淀四个小时。从 20%盐水悬浮液中可以定量回收病毒，但从 1%盐水悬浮液中不能回收。添加 10%正常血清可从 1%悬浮液中定量回收病毒。通过描述的方法，从 4 个人粪便中检测到的 2 个粪便中，通过脑内接种法回收了病毒。