Lehtimäki T
Department of Biomedical Sciences, University of Tampere, Finland.
Clin Chim Acta. 1991 Dec 16;203(2-3):177-82. doi: 10.1016/0009-8981(91)90289-o.
In order to assess the validity of Apo E phenotyping from stored specimens, phenotypes determined from fresh serum samples were compared with those from stored (8 years at -20 degrees C) samples from the same individuals (n = 42). The effect of early postmortem period on Apo E phenotype determinability was studied by taking four duplicate blood samples from eight cadavers 2, 6, 12, and 24 h after death. Apo E phenotyping was performed directly from serum by isoelectric focusing and immunoblotting. From the cadavers, the same Apo E phenotypes were obtained 2, 6, 12, and 24 h after death. After eight years' storage five out of ten Apo E4/4 phenotypes were falsely recorded as Apo E4/3 and one out of six Apo E4/3, one out of 12 Apo E4/2 were falsely interpreted as Apo E3/3. Phenotypes Apo E2/2 (n = 2), Apo E3/2 (n = 10), and Apo E3/3 (n = 2) were correctly assessed after 8 years of storage. In the total material, 17% (7/42) of Apo E phenotypes were incorrectly assessed after the storage.
为了评估从储存样本中进行载脂蛋白E（Apo E）表型分析的有效性，将新鲜血清样本所确定的表型与来自相同个体（n = 42）的储存样本（在-20℃下储存8年）的表型进行比较。通过在8具尸体死亡后2、6、12和24小时采集四份重复血样，研究死后早期对Apo E表型可测定性的影响。通过等电聚焦和免疫印迹法直接从血清中进行Apo E表型分析。从尸体上，在死亡后2、6、12和24小时获得了相同的Apo E表型。储存八年后，十分之五的Apo E4/4表型被错误记录为Apo E4/3，六分之一的Apo E4/3、十二分之一的Apo E4/2被错误地解释为Apo E3/3。Apo E2/2（n = 2）、Apo E3/2（n = 10）和Apo E3/3（n = 2）表型在储存8年后被正确评估。在全部样本中，储存后17%（7/42）的Apo E表型被错误评估。
