Hommes F A, Varghese M
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
Clin Chim Acta. 1991 Dec 16;203(2-3):211-24. doi: 10.1016/0009-8981(91)90293-l.
Urinary oligosaccharides can be separated by high-performance anion-exchange chromatography using a Dionex CarboPac PA1 column, elution with aqueous sodium hydroxide and sodium acetate solutions and detection by pulsed amperometry. Each of the urines of patients with glycoprotein degradation disorders yielded a pattern of oligosaccharide excretion unique for that disorder, facilitating an unambiguous diagnosis. The method is sensitive (10 microliters of urine required) and fast (40 min).
尿寡糖可以通过使用戴安公司的CarboPac PA1柱的高效阴离子交换色谱法进行分离，用氢氧化钠水溶液和醋酸钠溶液洗脱，并通过脉冲安培法进行检测。患有糖蛋白降解障碍的患者的每一份尿液都产生了一种该疾病特有的寡糖排泄模式，有助于明确诊断。该方法灵敏（只需10微升尿液）且快速（40分钟）。
