延伸因子EF-Tu（EF-1α）与SUP 2蛋白之间的结构比较及进化关系。

Structure comparison and evolutionary relations between elongation factors EF-Tu (EF-1 alpha) and SUP 2 proteins.

作者信息

Samsonova M G, Inge-Vechtomov S G, Taylor P

机构信息

Dept. of Genetics, Leningrad State University, USSR.

出版信息

Genetica. 1991;85(1):35-44. doi: 10.1007/BF00056104.

DOI:10.1007/BF00056104

PMID:1778473

Abstract

On the basis of high homology and structural similarity, three genes, SUP2 Saccharomyces cerevisiae, SUP2 Pichia pinus and GST1 Homo sapiens, might be considered as members of one family named SUP2. Comparison of the primary structure of SUP2 proteins and elongation factors EF-Tu(EF-1 alpha) from 19 different species was performed. It was found that SUP2 proteins bear more homology to eukaryotic elongation factor than to procaryotic EF-Tu, though the degree of sequence conservation in SUP2 proteins is smaller than in EF-1 alpha factors. The extensive phylogenetic analysis of SUP2 and EF-Tu(EF-1 alpha) genes was performed by means of 3 methods, 2 phenetic and one cladystic (maximal parsimony). The data support the close relation of SUP2 genes to other elongation factor genes.

摘要

基于高度同源性和结构相似性，三个基因，即酿酒酵母的SUP2、松树毕赤酵母的SUP2和智人的GST1，可能被视为一个名为SUP2的家族成员。对来自19个不同物种的SUP2蛋白一级结构与延伸因子EF-Tu（EF-1α）进行了比较。结果发现，SUP2蛋白与真核延伸因子的同源性高于与原核EF-Tu的同源性，尽管SUP2蛋白的序列保守程度低于EF-1α因子。通过三种方法（两种表型方法和一种分支方法（最大简约法））对SUP2和EF-Tu（EF-1α）基因进行了广泛的系统发育分析。数据支持SUP2基因与其他延伸因子基因的密切关系。