Kushnirov V V, Ter-Avanesian M D, Smirnov V N, Chernov Iu O, Derkach I L, Novikova O N, Inge-Vechtomov S G, Neĭstat M A, Tolstorukov I I
Mol Biol (Mosk). 1990 Jul-Aug;24(4):1024-36.
SUP2(SUP35) is an omnipotent suppressor gene, coding for an EF-1 alpha-like protein factor, involved in the control of translational accuracy in yeast Saccharomyces cerevisiae. A SUP2 gene analogue from yeast Pichia pinus was isolated by complementation of temperature-sensitive sup2 mutation of S. cerevisiae. Nucleotide sequence of the SUP2 gene of P. pinus codes for a protein of 82.4 kDa exceeding the SUP2 protein of S. cerevisiae for 6 kDa. The SUP2 gene product of P. pinus is similar to the Sup2 protein of S. cerevisiae by its structure and includes a highly conservative (76%) C-terminal region homologus to EF-1 alpha and a lowly conservative N-terminal region. The relation between the evolutionary conservativity of different regions of the Sup2 protein and their functional significance is discussed.
SUP2（SUP35）是一种全能抑制基因，编码一种类似于EF-1α的蛋白质因子，参与酿酒酵母翻译准确性的控制。通过互补酿酒酵母温度敏感型sup2突变，从毕赤酵母中分离出一个SUP2基因类似物。毕赤酵母SUP2基因的核苷酸序列编码一种82.4 kDa的蛋白质，比酿酒酵母的SUP2蛋白质大6 kDa。毕赤酵母的SUP2基因产物在结构上与酿酒酵母的Sup2蛋白质相似，包括一个与EF-1α高度保守（76%）的C末端区域和一个保守性较低的N末端区域。文中讨论了Sup2蛋白质不同区域的进化保守性与其功能意义之间的关系。
