Knorre D G, Vlassov V V
Institute of Bioorganic Chemistry, Siberian Division, USSR Academy of Sciences, Novosibirsk.
Genetica. 1991;85(1):53-63. doi: 10.1007/BF00056106.
Development of efficient methods for synthesis of oligonucleotides and oligonucleotide analogs has opened up the possibility of designing a broad spectrum of affinity reagents for specific modification of nucleic acids and proteins. These affinity reagents are used for investigation of the topology of ribosomes and nucleic acid polymerases. Oligonucleotides and their analogs are already used for suppression of specific gene expression and for elucidation of the physiological role of their products. Oligonucleotide derivatives appear to offer considerable promise as potential gene-targeted drugs such as antivirals and specific inhibitors of oncogene expression.
高效合成寡核苷酸及寡核苷酸类似物方法的发展，为设计一系列用于核酸和蛋白质特异性修饰的亲和试剂开辟了可能性。这些亲和试剂用于研究核糖体和核酸聚合酶的拓扑结构。寡核苷酸及其类似物已被用于抑制特定基因表达以及阐明其产物的生理作用。寡核苷酸衍生物作为潜在的基因靶向药物，如抗病毒药物和癌基因表达的特异性抑制剂，似乎具有相当大的前景。
