Sequence-specific chemical modification of double-stranded DNA with alkylating oligodeoxyribonucleotide derivatives.

作者信息

Vlassov V V, Gaidamakov S A, Zarytova V F, Knorre D G, Levina A S, Nikonova A A, Podust L M, Fedorova O S

机构信息

Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of U.S.S.R., Novosibirsk.

出版信息

Gene. 1988 Dec 10;72(1-2):313-22. doi: 10.1016/0378-1119(88)90158-8.

Chemical modification of double-stranded (ds) DNA with alkylating oligodeoxynucleotide (oligo) derivatives, 5'-p(N-2-chloroethyl-N-methylamino) benzylamides of oligos, has been investigated. In contrast to relaxed plasmid DNAs, the superhelical molecules interact with the oligo derivatives and specific alkylation of the DNAs occurs at the regions complementary to the oligo reagents. Alkylating derivatives of oligocytidylates and pT(pCpT)6 react with corresponding homopyrimidine-homopurine tracts within ds DNA fragments due to triple helix formation.