Conrad Karsten, Schneider Hauke, Ziemssen Tjalf, Talaska Thomas, Reinhold Dirk, Humbel Rene-Louis, Roggenbuck Dirk
Institute of Immunology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Fetscherstrasse 74, 01307 Dresden, Germany.
Ann N Y Acad Sci. 2007 Aug;1109:256-64. doi: 10.1196/annals.1398.031.
A novel line immunoassay for the multiparametric detection of 11 antiganglioside autoantibodies (GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, and GQ1b) was evaluated by comparing the reactivities in sera of 77 patients with suspected or definite autoimmune peripheral neuropathies (PNP), 60 blood donors, and 30 systemic lupus erythematodes (SLE) patients. At least one antiganglioside autoantibody was detectable in 97.4% of the patients with neuropathies compared to 12.2% in the control group. A broad spectrum of reactivities with more than two antiganglioside autoantibodies including all tested gangliosides except GD2 and GT1b was found in nearly one-third of the patients with neuropathies whereas in the control group autoantibody profiles with more than two reactivities were observed in two SLE patients only. For the first time anti-GM4 IgG and IgM antibodies were shown in PNP including Guillain-Barré syndrome (GBS), Miller-Fisher syndrome (MFS), and multifocal motor neuropathy (MMN). Different autoantibody profiles detectable by this multiparametric assay may help to diagnose different entities within the growing spectrum of autoimmune PNP.
通过比较77例疑似或确诊自身免疫性周围神经病（PNP）患者、60例献血者和30例系统性红斑狼疮（SLE）患者血清中的反应性，对一种用于多参数检测11种抗神经节苷脂自身抗体（GM1、GM2、GM3、GM4、GD1a、GD1b、GD2、GD3、GT1a、GT1b和GQ1b）的新型线性免疫测定法进行了评估。与对照组的12.2%相比，97.4%的神经病患者可检测到至少一种抗神经节苷脂自身抗体。在近三分之一的神经病患者中发现了广泛的反应性，即与两种以上抗神经节苷脂自身抗体反应，包括除GD2和GT1b外所有检测的神经节苷脂，而在对照组中，仅在两名SLE患者中观察到具有两种以上反应性的自身抗体谱。首次在包括格林-巴利综合征（GBS）、米勒-费希尔综合征（MFS）和多灶性运动神经病（MMN）在内的PNP患者中发现了抗GM4 IgG和IgM抗体。通过这种多参数检测可检测到的不同自身抗体谱可能有助于诊断自身免疫性PNP不断扩大的范围内的不同实体。
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