Caudie Christiane, Vial Christophe, Petiot Philippe, Bouhour Françoise
Laboratoire d'immunologie, Hôpitaux de Lyon, Centre hospitalier Lyon Sud, Pierre-Bénite.
Ann Biol Clin (Paris). 2010 Nov-Dec;68(6):675-80. doi: 10.1684/abc.2010.0495.
We evaluated the analytical performances of a new line immunoassay (LIA) for the simultaneous detection of twelve anti-ganglioside and anti-sulfatide autoantibodies from Generic Assays, in comparison with our dot immunoassay. The LIA detected IgG and/or IgM autoantibodies against human GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, GQ1b and sulfatide. Forty sera of patients with IgG autoantibody profiles in acute autoimmune neuropathies and 39 sera of patients with IgM autoantibody profiles in chronic autoimmune neuropathies were tested. To have a better sensitivity, 20 μL of sera instead of 10 μL were used. In these conditions, we observed a good concordance of IgG and IgM autoantibody profiles by both immunoassays. The test including novel gangliosides was easy to perform and superior for identifying autoimmune neuropathies. The data indicate that the test provides reliable simultaneous detection of autoantibodies against a large panel of human gangliosides and sulfatide with a good diagnostic usefulness in combination with clinical and electrophysiological data.
我们评估了一种新型线性免疫测定法（LIA）的分析性能，该方法用于同时检测通用测定法中的十二种抗神经节苷脂和抗硫脂自身抗体，并与我们的斑点免疫测定法进行了比较。LIA检测针对人GM1、GM2、GM3、GM4、GD1a、GD1b、GD2、GD3、GT1a、GT1b、GQ1b和硫脂的IgG和/或IgM自身抗体。对40例急性自身免疫性神经病患者的IgG自身抗体谱血清和39例慢性自身免疫性神经病患者的IgM自身抗体谱血清进行了检测。为了获得更好的灵敏度，使用了20μL血清而非10μL。在这些条件下，我们观察到两种免疫测定法的IgG和IgM自身抗体谱具有良好的一致性。包含新型神经节苷脂的检测易于操作，在识别自身免疫性神经病方面更具优势。数据表明，该检测方法能够可靠地同时检测针对一大类人神经节苷脂和硫脂的自身抗体，结合临床和电生理数据具有良好的诊断价值。
