Comparative study of the glial fibrillary acidic protein (GFAP) in toad, kitten and cat sensory ganglia.

The peroxidase-antiperoxidase (PAP) method was used on paraffin embedded tissue to demonstrate the presence of the glial fibrillary acidic protein (GFAP) in sensory ganglia of kittens, cats and toads. Special attention was paid to the influence of fixation. It appeared that the final immunostaining of GFAP depends on the fixative and on the species used. GFAP was found in cat sensory ganglia at birth and at all subsequent stages studied. The satellite cells from toad sensory ganglia were also positive, although they displayed a weaker immunoreactivity. The amphibian material showed a clear sensitivity to the fixative used.