Nomura H, Iwakawa S, Saito Y, Hori R, Okumura K
Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Japan.
J Pharmacobiodyn. 1991 Sep;14(9):527-31. doi: 10.1248/bpb1978.14.527.
The effect of experimental ulcers on the binding of human epidermal growth factor (hEGF), beta-urogastrone, to plasma membranes isolated from rat gastric mucosa was studied in order to develop hEGF as an anti-ulcer drug. The binding of [125I]hEGF to the gastric plasma membranes was significantly decreased 1 h after treatment with aspirin (300 mg/kg) and after 12 h of water-immersion stress. However, the number of EGF binding sites was increased 12 h after aspirin administration. There was little change in the binding of [125I]insulin to the gastric plasma membranes in response to water-immersion stress. These results indicate that changes in EGF binding to its receptors occur on plasma membranes of the rat gastric mucosa during ulcer.
为了将人表皮生长因子（hEGF），即β-尿抑胃素开发成一种抗溃疡药物，研究了实验性溃疡对从大鼠胃黏膜分离的质膜结合hEGF的影响。在用阿司匹林（300mg/kg）处理1小时后以及水浸应激12小时后，[125I]hEGF与胃质膜的结合显著降低。然而，阿司匹林给药12小时后，表皮生长因子（EGF）结合位点的数量增加。水浸应激后，[125I]胰岛素与胃质膜的结合几乎没有变化。这些结果表明，在溃疡形成过程中，大鼠胃黏膜质膜上EGF与其受体的结合发生了变化。
