Tamura M, Monson M C, Davis B, Mamalis N
Department of Ophthalmology, University of Utah School of Medicine, Salt Lake City.
Jpn J Ophthalmol. 1991;35(2):194-206.
An animal model was used to evaluate the influence of residual lens cortex on postoperative inflammation and cellular deposits on intraocular lenses (IOLs). Eighteen New Zealand white rabbits underwent bilateral endocapsular phacoemulsification with placement of a one-piece polymethylmethacrylate (PMMA) IOL in the capsular bag. A predetermined amount (15 mg, 30 mg or 60 mg) of homogenized lens cortex was injected into the anterior chamber of one of the eyes of each rabbit after bilateral IOL implantation. The contralateral eye served as the control. The animals underwent slit-lamp examination postoperatively, and the anterior segment of each eye was examined by gross and light microscopy. On slit-lamp examination, the eyes which had received a 60-mg injection of cortex showed a significantly higher amount of posterior synechiae than their contralateral, control eyes. After 2 weeks of observation, the rabbits were sacrificed, the eyes were enucleated, and the IOLs were removed and examined by light microscopy and scanning electron microscopy (SEM). The number of cellular deposits on the IOLs removed from the eyes receiving a 30-mg injection of cortex was significantly larger than that found in their contralateral, control eyes (P less than 0.05), but the eyes in the 60-mg and 15-mg injection groups showed no significant difference between control eyes and IOL eyes in the number of cellular deposits on explanted IOLs. Observation by SEM determined that the filopodia on the epithelioid cells found on the IOLs removed from the eyes in the 60-mg injection group showed an increased number and length compared to their controls. The epithelioid cells on the IOLs of the 30-mg injection group showed an increased number of filopodia compared to their controls.
采用动物模型评估残留晶状体皮质对术后炎症及人工晶状体（IOL）上细胞沉着物的影响。18只新西兰白兔接受双侧囊内超声乳化术，并在囊袋内植入一片式聚甲基丙烯酸甲酯（PMMA）人工晶状体。在双侧植入人工晶状体后，向每只兔子的一只眼的前房注射预定剂量（15毫克、30毫克或60毫克）的匀浆晶状体皮质。对侧眼作为对照。术后对动物进行裂隙灯检查，并用大体和光学显微镜检查每只眼的前段。在裂隙灯检查中，接受60毫克皮质注射的眼比其对侧对照眼出现的后粘连明显更多。观察2周后，处死兔子，摘除眼球，取出人工晶状体，并用光学显微镜和扫描电子显微镜（SEM）检查。从接受30毫克皮质注射的眼中取出的人工晶状体上的细胞沉着物数量明显多于其对侧对照眼（P小于0.05），但60毫克和15毫克注射组的眼中，对照眼和植入人工晶状体的眼中，取出的人工晶状体上的细胞沉着物数量无显著差异。扫描电子显微镜观察确定，与对照相比，60毫克注射组的眼中取出的人工晶状体上发现的上皮样细胞的丝状伪足数量和长度增加。30毫克注射组的人工晶状体上的上皮样细胞的丝状伪足数量比其对照增加。
