Kimura M, Koizumi S, Otsuka F
Methods Enzymol. 1991;205:114-9. doi: 10.1016/0076-6879(91)05093-b.
A sensitive method for detecting metallothioneins (MTs) by using silver staining and autoradiography after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of carboxymethylated MTs is described. Carboxymethylation of MTs is indispensable because it prevents their aggregation, thereby allowing each of them to appear as a single band using SDS-PAGE. Metallothioneins can be detected with a limit of nanogram levels per lane. This method can be applied to MTs induced in in vitro cultured cells and in in vivo tissues.
本文描述了一种灵敏的检测金属硫蛋白（MTs）的方法，该方法是在对羧甲基化的MTs进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）后，通过银染和放射自显影来实现的。MTs的羧甲基化是必不可少的，因为它可以防止其聚集，从而使它们在SDS-PAGE中各自呈现为一条单一的条带。金属硫蛋白的检测限可达每条泳道纳克水平。该方法可应用于体外培养细胞和体内组织中诱导产生的MTs。
